摘要
以耐冷水稻品系996和冷敏感水稻品系4628为材料,在抽穗开花期采用人工气候室进行连续7 d低温(06:00—08:00,19℃;08:00—10:00,21℃;10:00—16:00,23℃;16:00—19:00,21℃;19:00—23:00,19℃;23:00—06:00,17℃)和适温(06:00—08:00,24℃;08:00—10:00,26℃;10:00—16:00,30℃;16:00—19:00,26℃;19:00—23:00,24℃;23:00—06:00,22℃)处理,研究低温胁迫对水稻剑叶保护酶活性和膜透性的影响。结果表明:低温胁迫下水稻剑叶中超氧化物歧化酶(SOD)、过氧化物酶(POX)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(AsA-POX)活性呈先升后降趋势;随低温胁迫时间延长,膜脂过氧化产物硫代巴比妥酸反应产物(TBARS)和过氧化氢(H2O2)、超氧阴离子(O2.-)含量迅速上升,膜透性增加;品系996在低温胁迫下其剑叶中保护酶类活性显著高于品系4628,而O2.-、H2O2和MDA含量和膜透性却显著低于品系4628;低温胁迫下水稻剑叶保护类酶维持较高的活性,降低O2.-、H2O2、TBARS含量和膜透性,是品系996耐低温的生理基础。
The experiments were carried out in growth chambers to investigate the effects of low temperature stress during flowering period on protective enzyme activities and membrane permeability of flag leaves using two rice cultivars including low-temperature tolerant cultivar 996 and low-temperature sensitive cultivar 4628. Optimal temperature（06：00-8：00, 24 ℃ ; 08：00-10：00, 26 ℃ ; 10：00-16：00, 30 ℃ ; 16：00-19：00, 26 ℃ ; 19：00-23：00, 24 ℃ ; 23：00-06：00, 22℃）and low temperature（06：00-8：00, 19 ℃ ; 08：00-10：00, 21 ℃ ; 10：00-16：00, 23 ℃ ; 16：00-19：00, 21 ℃ ; 19：00-23：00, 19 ℃ ; 23：00-06：00, 17 ℃）were respectively treated for seven days as two treatments after heading. The results showed that the activity of superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POX） and ascorbic acid-peroxidase （AsA-POX） in flag leaves of two rice cultivars increased at early stage of low temperature stress, then decreased. The contents of thiobarbituric acid reaction substance（TBARS） and hydrogen peroxide（H2O2）, superoxide anion（O2·^-） and the membrane permeability were increased remarkably as the period of exposure to low temperature lengthened. The protective enzyme activities in the cultivar 996 were higher while the contents of O2·^-, H2O2 and TBARS and the membrane permeability were lower than those in the cultivar 4628 under low temperature stress. Therefore, higher protective enzyme activities, which may resulted in lower contents of H2O2, O2·^-and TBARS and lower membrane permeability in rice flag leaf were the physiological basis of low-temperature tolerant rice cultivar.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第6期581-585,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(31101134)
湖南省自然科学基金项目(10JJ3091)
湖南农业大学作物种质创新与资源利用国家重点实验室培育基地开放基金项目(10KFXM10)
湖南农业大学作物栽培学与耕作学学科青年基金项目
关键词
水稻
开花期
低温胁迫
剑叶
保护酶
膜透性
rice
flowering stage
low temperature stress
flag leaf
protective enzyme
membrane permeability
