摘要
目的建立鬼臼毒素固体脂质纳米粒含量的高效液相色谱测定方法。方法以ODS-C18（4．6mm×250mm，5μm）为分析柱，以甲醇-水-冰醋酸（59：41：0，6）为流动相，流速0．8mL·min^-1，检测波长为285nm，进样量20μL，柱温26℃。结果当鬼臼毒素浓度在1．6～80．0μg·mL^-1。范围内时，峰面积与浓度之间呈良好的线性关系（r=0．9999，n=5），高、重、低3个剂量的平均回收率分别为99．09％，99．88％，100．92％。日内和日间精密度RSD分别为0．39％，0．42％，3批鬼臼毒素固体脂质体中鬼臼毒素的含量分别为94．89％，94．76％，97．24％。结论该方法准确，简便，灵敏，重现性好，适合测定鬼臼毒素的含量。
Objective To establish the HPLC method for the determination of podophyllotoxin in pod-sin. Methods ODS-C18 （4.6 mm×250 mm, 5 μm） was used as the stationary phase. The mobile phase was the solution containing methanol-water-glacial acetic acid （59 ： 41 ： 0.6）; flow rate： 0.8 mL·min^-1; wavelength of UV detection： 285 nm, sample： 20 μL and temperature of column： 26 ℃. Results The calibration curves was linear in the concentrations of 1.6～80.0 μg·L^-1 （r =0.999 9, n = 5）. The average recovery of three doses was 99.09%, 99.88%, 100.92%; the contents of podophyllotoxin in 3 batches pod-sin were 94.89%, 94.76%, 97.24%, respectively. Conclusion The method is simple, accurate, rapid and reproducible. It is suitable to assay the content of the drug.
出处
《医药导报》
CAS
2006年第9期953-954,共2页
Herald of Medicine
