摘要
目的:建立疣克酊中鬼臼毒素含量的高效液相色谱测定方法。方法:以Discovery C_(18)(4.6mm×250mm,5μm)为分析柱,以甲醇-水(50:50)为流动相,检测波长285nm,流速为0.8mL·min^(-1)。结果:当鬼臼毒素的浓度在24.8～99.2 mg·L^(-1)范围内时,面积与浓度间呈良好的线性关系(r=0.9999,n=6),方法的平均回收率为98.5％,日内和日间精密度分别为3.8％和4.1％。3批疣克酊剂中鬼臼毒素的百分含量分别为102.00％,101.46％,100.39％。结论:本方法具有准确,简便,灵敏且重现性好的特点,可适用于复方制剂中鬼臼毒素含量的测定。
OBJECTIVE To establish HPLC method for the determination of podophyllotoxin in Youkeding tincture. METHODS The Discovery C18(4.6 mm×150 mm,5 μm)was used as analytical column. The mobile phase was methanol-water(50:50). Detection wavelength was 285 run. The flow rate was 0. 8 mL·min . RESULTS The calibration curves was linear in the concentration range of 24.8-99.2 mg·L-1 ( r = 0. 9999, n = 6). The average recovery was 98.5 % ( RSD = 0.48 % ). The contents of podophyllotoxin in 3 batches of Youkeding tincture were 102.00%, 101.46%, 100.39% respectively. CONCLUSIONS The method is simple,accurate, sensitive and reproducible. It is suitable for the determination of podophyllotoxin and its preparations.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第12期721-722,共2页
Chinese Journal of Hospital Pharmacy
