摘要
目的观察一氧化氮供体偶联的阿司匹林衍生物Ⅱ6对血栓形成的影响,并探讨其作用机制。方法以阿司匹林为对照,观察Ⅱ6经口给药对大鼠下腔静脉血栓和脑血栓形成的影响,采用放射免疫法分析Ⅱ6对大鼠血浆、主动脉条和ECV304细胞上清液中血栓素B2(TXB2)和6-酮-前列腺素F1α(6-Keto-PGF1α)含量的影响,用G riess法测定Ⅱ6对ECV304细胞外液和大鼠血浆中一氧化氮(NO)释放的影响。结果化合物Ⅱ6可减轻实验动物血栓的重量,降低TXB2的生成,提高大鼠血清和ECV304细胞上清液中NO的含量。结论Ⅱ6具有较好的抗血栓作用,其作用机制可能与抑制TXA2的释放,增加NO含量有关。
Aim To study the inhibitory effect of NO donating-aspirin derivative Ⅱ 6 on thrombosis and its mechanism of nitric oxide release. Methods Inferior vena cava thrombosis and cerebral thrombosis model in rats were used to investigate the antithrombotic activities of Ⅱ6. TXB2 and 6-Keto-PGF1α in rat serum, rat aortic strip and in ECV304 cells were determined by radioimmunosay. NO content in the serum of rats and extracellular fluid of the ECV304 cells were tested with Griess reagent. Results Ⅱ 6 inhibited the thrombosis in inferior vena cava and brain, and it reduced the concentration of TXB2. but increased NO production. Conclusion Ⅱ 6 has an inhibitory effect on thrombosis and increases NO content in vivo and vitro.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第7期840-844,共5页
Chinese Pharmacological Bulletin
