摘要
用比浊法和放射免疫分析技术研究甲基莲心碱（Ｎｅｆ）抗血小板聚集作用及其对ＴＸＡ２／ＰＧＩ２与ｃＡＭＰ／ｃＧＭＰ浓度的影响。结果显示，Ｎｅｆ在体外明显抑制ＡＤＰ，胶原，ＡＡ及ＰＡＦ诱导的家兔血小板聚集，ＩＣ５０分别为１６，２２，１９３及１０３μｍｏｌ·Ｌ－１；Ｎｅｆ明显抑制ＡＡ诱导的血小板ＴＸＡ２的生成和释放，对动脉环ＰＧＩ２的生成有促进作用；Ｎｅｆ剂量依赖性地升高血小板ｃＡＭＰ浓度，对ｃＧＭＰ无明显影响。结果提示Ｎｅｆ抗血小板聚集作用的机理与抑制ＴＸＡ２生成，增加血管ＰＧＩ２及血小板ｃＡＭＰ含量有关。
Neferine (Nef), a hypotensive agent with antiarrythmic action, is a dibenzyl isoquinoline alkaloid isolated from a Chinese medicinal herb( Nelumbo nucifera Gaertn). Its effects on platelet aggregation and TXA 2/PGI 2 and cAMP/cGMP balance were studied with methods of turbidimetry and RIA. Nef was shown to significantly inhibit rabbit platelet aggregation induced by ADP, collagen, arachidonic acid (AA) and platelet-activating factor(PAF) with IC 50 of 16, 22, 193 and 103 μmol·L -1 , respectively. Nef was found to increase vascular 6 keto PGF 1α and platelet cAMP levels in dose dependent manner, but inhibit AA stimulated TXA 2 release from platelets. Nef showed no significant effect on the platelet cGMP level. The results suggest that the mechanism of Nef on platelet aggregation is related to regulation of TXA 2/PGI 2 and cAMP/cGMP balance.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第1期1-4,共4页
Acta Pharmaceutica Sinica
