摘要
局部用氯化铁损伤血管以造成动物大脑中动脉血栓，于血栓形成后２４ｈ观察大鼠脑梗塞范围、行为及脑病理改变。结果表明，本模型造成的脑梗塞及行为障碍特点与Ｔａｍｕｒａ等（１９８ｌ）［１］建立的大鼠局部脑缺血模型相一致。所造成的血栓为混合血栓。本研究还观察了阿司匹林对脑血栓的预防作用及尿激酶的治疗作用。阿司匹林及尿激酶均可降低脑梗塞范围，改善行为障碍。结果表明该模型既可以评价溶栓因子又可以检验抗栓因子，为研究抗血栓药物提供了一种较好的途径。
The purpose of this study was to develope a rat model of cerebral
artery thrombosisby adding FeCl3 to the surface of right middle
cerebral artery(MCA) for 30 min. After 4 h,8h, 24h,48 h, 72 h, 1
week and 2 week,the extent of neurologic deficits of rats was
evaluated and after 24h and 48 h the size of cerebral infarction was
measured. The results indicate that the features ofneurologic
deficits and infarction were similar to those of the rat focal
cerebral ischemia model estab-lished by Tamura et al(198l)[1].The
nature of thrombus formed in our experiment is combined-thrombus. The
antithrombotic effect of aspirin and the thrombolytic effect of
urokinase were alsoexamined. Aspirin(50 mg·kg-1)was given by
duodenum 30 min before adding FeCl3 to the surfaceof the right MCA
and urokinase(4000 U·kg-1, iv) was given 30 min after thrombosis.
After opera-tion, the behavior of rats were observed. The cerebral
infarction was evaluated 24 h after thrombosis.The infarcted areas in
the aspirin and the urokinase groups were reduced to 20%and 11%those
of thecontrol group, respectively.The neurologic deficits were also
attenuated. These studies suggest thatthis rat medel may be used for
testing not only antithrombotic but also thrombolytic agents.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第9期662-667,共6页
Acta Pharmaceutica Sinica
关键词
抗血栓
尿激酶
脑血栓形成
动物模型
乙酰水杨酸
Model of middle cerebral artery thrombosis
Antithrombotic
effect
Thrombolyticeffect
Aspirin
Urokinase
