摘要
目的:检测鼻咽癌中信号传导与转录激活因子Stat3及其靶基因产物与VEGF的表达,探讨其在鼻咽癌发生、发展中的作用。方法:采用免疫组化技术检测慢性鼻咽炎症29例,鼻咽癌活检组织60例,鼻咽癌颈部淋巴结转移28例中Stat3、CyclinD1、Bcl-xL和VEGF蛋白的表达。结果:鼻咽癌组中Stat3及其靶基因产物(Bcl-xLCyclinD1)、VEGF的表达明显高于鼻咽粘膜慢性炎症组;鼻咽癌与鼻咽癌颈部淋巴结转移灶中Stat3及其靶基因产物表达比较无统计学意义。鼻咽癌及鼻咽癌颈部淋巴结转移灶中Stat3、CyclinD1与VEGF的表达有显著相关性,而Bcl-xL和VEGF的表达无显著相关性。结论:Stat3及其靶基因产物与VEGF可能在鼻咽癌的发生、发展中发挥重要作用。二者之间的相关性表明VEGF基因可能由Stat3蛋白调节。
Objective： To investigate the expression of Star3, the signal transducer and activator of transcription factor 3, and its downstream targets, including BCL-XL, Cyclin D1 and VEGF, in nasopharyngeal carcinoma （NPC） and to explore the mechanism of tumorigenesis of NPC. Methods： The expression of Stat3, BCL-XL, Cyclin D1 and VEGF proteins in paraffin-embedded specimens of 29 cases with chronic nasopharyngitis, 60 cases of NPC and 28 cases of lymph node metastasis was studied using immunohistochemical staining （ABC）. Results： The positive rate of Star3, BCL-XL, Cyclin D1 and VEGF in chronic nasopharyngitis was 41.38% （12/29）, 34.48% （10/29）, 27.59% （8/29） and 24.13% （7/29）, respectively; the expression of Stat3, BCL-XL, Cyclin D1 and VEGF in nasopharyngeal carcinoma was 73.33% （44/60）, 68.33% （41/60）, 55.00 % （33/60） and 80.00% （48/60）, respectively, with significant differences when NPC was compared to BCL-XL, Cyclin D1 and VEGF in lymph node chronic nasopharyngitis; the positive rate of Stat3, metastasis was 85.71% （24/28）, 75.00% （21/28）, 71.43% （20/28） and 82.14% （23/28）, respectively, with significant differences when lymph node metastasis was compared to chronic nasopharyngitis. There was no significant difference in the expression of these 4 proteins between NPC and lymph node metastasis. Spearman correlation analysis showed ＂there was a significant correlation between the expression of Stat3, Cyclin D1 and VEGF in the NPC and lymph node metastasis. Conclusions： Stat3 and VEGF may play an important role in the development of NPC, and the VEGF gene may be directly regulated by Stat3 protein.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2006年第15期858-861,共4页
Chinese Journal of Clinical Oncology
基金
广东省卫生厅科研基金资助(编号:A2001466)
