摘要
目的:通过检测肺癌组织芯片中Stat3、bcl-2和VEGF的表达,探讨它们在肺癌的发生发展及浸润转移中的作用,为肺癌的早期诊断、预后判断提供一定的理论依据。方法:应用组织芯片技术及免疫组织化学方法检测正常肺组织、癌前病变、原发癌及淋巴结转移癌组织中Stat3、bcl-2和VEGF的表达情况,并分析与各临床病理参数之间的关系。结果:实验组中Stat3、bcl-2、VEGF的表达阳性率分别为65.17%、77.53%、79.77%,均显著高于正常对照组(P<0.05);Stat3表达阳性率与组织学分级、临床分期及淋巴结转移有关(均P<0.05);肺癌中Stat3与VEGF,bcl-2的表达呈明显正相关。结论:Stat3与肺癌的浸润和转移密切相关,因而Stat3可用于判断肿瘤预后的指标检测。
Objective ： To explore the roles of Stat3 ,bcl-2 and VEGF in occurrence ,development of lung cancer,by detecting the expression of them in a lung cancer tissue microarray and to supply a theoretical evidence for diagnosis and judgment of prognosis. Methods：We applied tissue microarray technique and SP {mmunohis tochemical method to detect Stat3,hcl-2 and VEGF protein expressions and analyzed their relationship with each other in a lung cancer tissue microarray containing normal lung tissue specimen,precancerous diseases, cancer specimen and metastasis specimen. Re-sults：Stat3,bcl-2 and VEGF proteins were respectively detected in 65.17% ,77.53% and 79. 77%of all the 89 lung cancer tissues,which were higher than those of control group,and the differences were significant （P〈0.05）. The positive ratio of the expressions on Stat3 were significantly relate to histological differentiations, clinical stages and lymphoid metastasis of lung cancers （P〈0.05）. There was significantly positive association among stat3,bcl-2 and VEGF. Conclu- sion ：Stat3 plays an important role in proliferation of tumor cells. Detected the marker will be important to estimate cancer progression.
出处
《中国误诊学杂志》
CAS
2007年第6期1179-1182,共4页
Chinese Journal of Misdiagnostics
