摘要
从外植体来源、生长调节剂种类、浓度等方面研究影响巴西蕉愈伤组织诱导的因素。结果表明,以未成熟雄花花序或球茎为外植体,MS+2,4-D4mg/L+IAA1mg/L+NAA1mg/L为愈伤组织诱导培养基,28℃下暗培养条件下,雄花花序的愈伤组织诱导率仅为1.7%,而以球茎为外植体的愈伤组织诱导率可达91.3%。
Banana （Musa spp.） is one of the most important food crops in the world. An attempt was made to study factors which affect induction of callus derived from the banana cultivar Brazil （Musa AAA Cavendish Subgroup cv. Brazil）, including source of explants, plant hormones, concentration of plant hormones and light intensity. Explants from immature male flowers and corms were cultured on the MS at the presence of 2,4-D 4 mg/L ＋ IAA 1 mg/L ＋ NAA 1 mg/L and incubated in the dark at 28℃. The callus inducing rates were 1.7% for immature male flowers and 91.3% for corms.
出处
《热带农业科学》
2006年第3期15-17,50,共4页
Chinese Journal of Tropical Agriculture
基金
国家自然科学基金项目(No.30260043)
博士学科专向科研基金项目(No.20050565004)资助
