摘要
勿忘我组培繁殖可通过外植体直接分化出不定芽和先形成愈伤组织后分化出不定芽途径。外植体有幼嫩小花序和叶片。在起始培养基中附加 2 0 mg/ L的苯甲酸钠对抑制细菌和真菌污染有一定的效果 ;适宜的起始培养基为 MS+ 1mg/ L 6 - BA+ 0 .2 mg/ L NAA+ 4 %蔗糖 + 0 .7%琼脂。增殖培养基以 MS+0 .4 mg/ L 6 - BA+ 0 .2 mg/ L NAA+ 3%蔗糖 + 0 .7%琼脂为好 ,每 30 d增殖倍数可达 5倍。生根培养基以1/ 2 MS+ 0 .4 mg/ L NAA+ 1.5 %蔗糖 + 0 .7%琼脂 ,同时附加 0 .0 2 mg/ L的 PP333效果为好 ,生根率可达96 % ,根明显增粗。试管苗移栽介质以木屑 +泥炭 +珍珠岩 (5∶ 2∶ 3)为好 ,移栽成活率最高可达 10 0 %。大棚栽培的试管苗生长正常 ,生产的切花与外植体来源的切花在形态性状上没有明显差异。
The adventitious buds can occur from explants or from callus of initiated explants, including young inflorescence and leaf. The contamination caused by bacteria and fungi can be effectively controlled on the initiated medium containing 20 mg/L sodium benzoate. The optimum medium for inducing adventitious buds from explants is MS with 1 mg/L 6 BA, 0.2 mg/L NAA, 3% sucrose and 0.7% agar. The optimum medium for shoot buds multiplication is MS with 0.4 mg/L 6 BA, 0.2 mg/L NAA, 3% sucrose and 0.7% agar, which makes the multiplication rate reach 5 times during 30 days. The optimum medium for rooting is 1/2 MS with 0.4 mg/L NAA, 1.5% sucrose, 0.7% agar and 0.02 mg/L PP333, which makes the rooting rate reach 96% and the roots be thicker than those on CK medium. The optimum medium for transplanting plantlets is wood crumb(5) : peat(2) : perlite(3), which makes the survival rate of plantlets reach 100%. The growth performance of plants obtained from tissue culture is normal in the plastid cover shed. There are no significant morphological difference between the flowers cut from regenerated plants and those cut from parental plants.
出处
《贵州农业科学》
CAS
2002年第1期9-13,共5页
Guizhou Agricultural Sciences
关键词
勿忘我
组织培养
快速繁殖
苯甲酸钠
L.sinuatum
tissue culture
rapid propagation
PP333
sodium benzoate
TDZ
