摘要
分别以3个香蕉品种的多芽体茎尖和5个香蕉品种的未成熟雄花为外植体,诱导产生胚性愈伤组织.结果分别从2个基因组类型为AAA的品种的多芽体茎尖和未成熟雄花获得了胚性愈伤组织,而未能从基因组类型为AAB或ABB的香蕉品种中获得胚性愈伤组织.胚性愈伤组织的诱导率受基因组类型、品种和培养条件等因素的影响.以上述4个品种的胚性愈伤组织为起始材料成功建立了胚性细胞悬浮系,不同香蕉品种,从其胚性愈伤组织建立胚性细胞悬浮系的难易程度有所不同.
Embryogenic callus was successfully induced starting from immature male flowers in 2 out of 5 banana cultivars and starting from scalps in 2 out of 3 cultivars. All these 4 cultivars from which embryogenic callus could be induced belonged to Musa AAA group. The frequency of embryogenic callus induction was depended on genotypes, cultivars and incubation conditions etc. Embryogenic cell suspensions (ECSs) were initiated successfully from embryogenic callus of all these 4 cultivars. The possibility of getting ECSs from embryogenic callus was also cultivar-dependent.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2004年第1期70-73,共4页
Journal of South China Agricultural University
基金
国家自然科学基金资助项目(39870434)
广东省自然科学基金资助项目(990714)
广东省科技攻关项目(C20209)
