摘要
目的:采用离子对反相高效液相色谱法测定六合维生素丸中烟酰胺、维生素B2、维生素B13种水溶性维生素的含量。方法:以C18为色谱柱,0.005mol/L庚烷磺酸钠溶液(含0.5%冰醋酸和0.05%三乙胺)-甲醇(72∶28)为流动相,流速为1ml/min,检测波长为260nm。结果:烟酰胺、维生素B2、维生素B1的线性范围分别为0.3154～15.7700μg、0.05227～2.6135μg、0.4474～22.3700μg;方法的回收率在97.5%～99.2%,RSD为1.3%～1.8%。结论:本法简便,快速,为六合维生素丸产品的质量控制及多维类药物中水溶性维生素含量的测定提供了可靠依据。
Objective：To develop an HPLC method for the determination of three vitamins B in six Vitamins Pills. Methods： C18 column was used. The mobile phase consisted of 0. 005 mol/L sodium heptanesulfonate （containing 0.5% glacial acetic acid and 0. 05% triethylamine） - methanol（72： 28）, the flow rate was 1.0ml/min. The UV detector wavelength was 260nm. Results：The calibration curves showed good linearity in the range of 0. 3154 - 15. 7700μg for nicotinamide,0. 05227-2. 6135μg for vitamin B2 ,0. 4474 -22. 3700μg for vitamin B~. Conclusion：This method developed is convenient and reliable and could be used for quality control of Six Vitamins Pills.
出处
《南通大学学报（医学版）》
2006年第4期255-256,共2页
Journal of Nantong University(Medical sciences)
