摘要
目的：建立以高效液相色谱法测定小儿四维葡钙颗粒中维生素B1、维生素B2含量的方法。方法：色谱柱为ZORBAX Eclipse XDB-C18柱（5μm,250mm×4.6mm）。流动相为0.005mol/L庚烷磺酸钠溶液（含0.5%冰醋酸和0.05%三乙胺）-甲醇（72∶28）,柱温30℃,流速为1ml/min,检测波长为260nm,进样量20μl。结果：维生素B1、维生素B2检测浓度的线性范围分别为7.13-22.96μg.mL-1（r=0.9991）、8.12-32.31μg.mL-1（r=0.9993）;平均回收率分别为101.1%（RSD=0.7%）、101.4%（RSD=0.6%）。结论：本方法简便准确、灵敏度高、结果可靠,可用于小儿四维葡钙颗粒中维生素B1、维生素B2的含量测定。
Objective Establish the method to determine vitamin B1 and vitamin B2 in Four Vitamin Glucose -Calcium particles by HPLC. Method PE Series 200 type HPLC is used ,which column is ZORBAX Eclipse XDB- C18（5μm, 250mm ×4. 6mm）, mobile phase is 0. 005mol/L heptane solution （0.5% acetic acid and 0.05% triethylamine） - methanol （72：28）. The detection wavelength was 260nm, flow rate is 1.0mL/min. Assay is external reference method. Result： A good linear correlation was obtained when vitamin Bl＇s concentration ranged from 7.13μg/mL to 22.96μg/mL（ r = 0. 9991 ） and vitamin B2＇s concentration ranged from 812μg/mL to 32. 31μg/mL（r = 0. 9993 ）. Recovery of B1 is 101.1%, RSD is 0. 7% ; Recovery of B2 is 101.4%, RSD is 0. 6%. Conclusion： This method is high - sensitive, separation effect and reproducibility are good, results are reliable, which can be applied to determine vitamin B1 and B2 in Four Vitamin Glucose - Calcium particles for children.
出处
《宜春学院学报》
2008年第4期89-90,共2页
Journal of Yichun University
