摘要
目的观察发育期大鼠反复惊厥后海马ATP酶与脑含水量的动态变化及川芎嗪干预对其的影响。方法162只20日龄健康SD大鼠随机分为3组:对照组、惊厥组及川芎嗪干预组。通过三氟乙醚反复吸入(连续6次,每天1次)制作发育期大鼠惊厥动物模型。取海马组织匀浆,检测各组动物反复惊厥后6 h1、d、3 d、7 d海马组织中Na+-K+-ATP酶、Ca2+-ATP酶的活性变化,同时观察脑含水量变化和光镜下海马区神经元病理改变。结果反复惊厥后6 h1、d3、d海马Na+-K+-ATP酶、Ca2+-ATP酶的活性均较对照组显著降低(P<0.01),伴随脑含水量显著升高(P<0.01),第7天三者与对照组相比较差异均无统计学意义(P>0.05)。脑含水量变化与海马Na+-K+-ATP酶、Ca2+-ATP酶的活性变化均呈显著负相关(前者r=-0.711,后者r=-0.673,P均<0.01)。川芎嗪干预组海马神经元水肿、变性坏死明显减轻,各时间点Na+-K+-ATP酶、Ca2+-ATP酶的活性较惊厥组显著升高(P<0.05或P<0.01),脑含水量显著降低(P<0.01)。结论Na+-K+-ATP酶、Ca2+-ATP酶的活性降低与惊厥性脑水肿和海马神经元迟发性损害密切相关,川芎嗪对发育期惊厥性脑损伤的保护作用可能与提高海马Na+-K+-ATP酶、Ca2+-ATP酶的活性有关。
Objective To investitage the effect of tetramethylpyrazine on the dynamic changes of ATPase activity in the hippocampus and water content of brain tissues of the developing rats following recurrent seizures： Methods 162 postnatal Sprague - Dawley rats of 20- day old were randomly divided into three groups： the control group, the seizure group and the tetramethylpyrazine treated group. Seizure model of rats were induced by inhalant flurothyl daily in 6 consecutive days. Brain tissue was sampled at 6 hours, 1 day, 3 days and 7days in each group after last seizure. We elevated the activities of Na^＋ - K^＋ - ATPase and Ca^2 ＋ - ATPase, and observed the water content of brain tissues and the pathological changes in the hippocampus of rat after recurrent seizures. Results Compared with controls, significant decrease in Na^＋ - K^＋ - ATPase and Ca^2＋ - ATPase activities were observed at 6 h,1 d,3 d after recurrent seizures（P 〈0.01）, while water content of brain tissues obviously increased （ P 〈 0.01 ）, no significant difference was found in three indexes between the two groups at post - seizure 7 d. Changes of Na^＋ - K^＋ - ATPase and Ca^2＋ - ATPase activities in seizure group showed a negative correlation with water content of brain tissues （the former r = - 0.711, the latter r = - 0.673, P〈0.01 ）. In tetramethylpyrazine treated group, edema, degeneration and necrosis of nerve cells in the hippocampus of the rat were significantly decreased, the activities of Na^＋ - K^＋ - ATPase and Ca^2＋ - ATPase in the hippocampus at different time points were much higher than those in seizure group（ P〈 0.05 or P〈0.01 ）, while water content of brain tissues was significandy lower than those in seizure group（ P〈0.01 ）. Conclusions There was close relationship between decreasing the activities of Na^＋ - K^ ＋ - ATPase and Ca^2 ＋ - ATPase in the hippocampus and increasing the water content of brain tissues and tardive neuron injury in the hippocampus. The protective effect of tetramethylpyrazine on the developing brain injury induced by seizures is probably related to raising the activities of Na^＋ - K^＋ - ATPase and Ca^2 ＋ - ATPase in the hippocampus.
出处
《中国急救医学》
CAS
CSCD
北大核心
2006年第7期518-520,T0001,共4页
Chinese Journal of Critical Care Medicine
基金
国家自然科学基金资助项目(No.30400483)
关键词
ATP酶
脑含水量
惊厥
海马
川芎嗪
ATPase
Water content of brain tissues
Seizure
Hippoeampus
Tetramethylpyrazine
