摘要
本研究目的为评价球花石斛多糖对正常小鼠免疫功能的影响。球花石斛采用碱提醇沉法得到球花石斛多糖,分别以400、600、800mg/kg小鼠灌胃给药连续10d。通过免疫器官重量检测,碳廓清试验,脾脏T、B淋巴细胞转化试验来观察其对免疫功能的影响。结果显示低、中剂量组的脾脏指数与对照组比较有显著性差异(P<0.01),中、高剂量组的吞噬指数、校正吞噬指数和B淋巴细胞增殖能力与对照组比较有显著性差异(P<0.01,P<0.05),表明球花石斛多糖可显著增加脾脏重量,增强巨噬细胞的碳廓清能力和B淋巴细胞的增殖能力。提示球花石斛多糖具有免疫增强作用,是一种良好的免疫调节剂。
The objective of this study was to evaluate the effects of Dendrobium thyrsiflorum Rehb.f polysaecharide （DTP）on immune function in normal mice. The DTP was obtained by alkali extraction and alcohol precipitation from Dendrobium thyrsiflorum Rehb.f and was administered on normal mice with 400 mg/kg,600 mg/kg and 800 mg/kg respectively through stomach for 10 d .The weight of immune organs,engulfing carbon granula test,T,B lymphocyte transformation test of mouse spleen cell were investigated to determin the effects of DTP on immune function. The results show that the spleen index in low and middle dosage treatment was higher than that of control group（ P 〈 0.01 ）. The macrophage engulfing carbon granula ability and B lymphocyte proliferation ability in middle and high dosage treatment are higher than that of control group（ P 〈 0.01, P 〈 0.05）. These findings indicate that DTP could significantly increase spleen weight,macrophage engulfing carbon granula ability and B lymphocyte proliferation capacity.So the conclusion is that DTP could enhance the immune function in mice andwould be a good immunomodulater.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第3期445-448,共4页
Natural Product Research and Development
基金
云南省教育厅科研基金项目(5J0344C)
关键词
球花石斛
多糖
免疫调节
碳廓清
淋巴细胞转化
Dendrobium thyrsiflorum Rchb. f
polysaccharide
immunomodulation
engulfing carbon granula
lymphocyte transformation
