摘要
本文将固定化酶柱，光纤以及停流技术结合起来，研究了以廉价而易得的硫胺素为荧光底物的葡萄糖的荧光分析，提出了一种快速、简便、精确地测定葡萄糖的新方法。其线性范围为1．0～60．0mg／L，线性相关系数为0．999，检测限为0．15mg／L。以5．0mg／L的葡萄糖作精度试验，RSD％＝2．1％（n＝11）。该方法已成功地用于血清中葡萄糖的测定，结果与光度法相符。
A new Fluorescence method for the determination of glucose has been proposed.It combines immobilized enzyme column, optical fiber and stopped-flow technique together.Thiamine of is used as a fluorimetric substrate which is cheap and easily available. The linear range of determinaiton is 1.0~60. 0 mg/L with a correlation coefficient of 0. 999 and a detection limit of 0. 15 mg/L. The method has been applied to the determination of glucose in human serum. Results are in agreement with those by spectrophotometry.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1996年第9期998-1001,共4页
Chinese Journal of Analytical Chemistry
