摘要
目的了解北京地区婴幼儿急性呼吸道感染与新近报道的人细小病毒（human bocavirus,HBoV）的关系.方法选择2003年11月至2004年2月收集的经间接免疫荧光和/或病毒分离排除了常见呼吸道病毒感染的急性呼吸道感染患儿标本319例,应用针对HBoV的NP1基因的PCR引物进行HBoV基因片段检测,随机选取HBoV基因检测阳性标本中的5例,对其PCR扩增产物直接进行核苷酸序列测定.将所测到的序列与GenBank中的基因序列进行比较分析.结果319例标本中HBoV基因检测阳性的为13例,其阳性检出率占本组检测标本的4.1%;HBoV阳性检出率在本组的毛细支气管炎患儿中最高,达10.9%（5/46）,其次为支气管炎患儿（6.3%,2/32）;HBoV检测阳性标本的患儿年龄主要分布于5个月～5岁,尤其是＜1岁的患儿;其中6～7个月的患儿中HBoV检测阳性的占50%（2/4）,9～10个月的患儿中阳性的占25%（2/8）,其次为5～6个月的患儿（18.2%）、11个月～1岁的患儿（14.3%）及8～9个月的患儿（12.5%）;在＜5个月的总计103例患儿中以及＞5岁的28例患儿中均未检测到HBoV阳性标本;基因序列分析表明,本研究中5例北京的HBoV之间基因序列的同源性在99.7%～100%之间;与st1、st2株的同源性为99.2%～99.4%.结论本研究结果提示北京地区部分儿科患者的急性呼吸道感染与HBoV有关,且HBoV感染在低年龄组儿童中更为常见.
Objective To investigate whether the novel parvovirus, human bocavirus（HBoV）, which was identified recently by two scientists in Sweden in September 2005, is related to acute respiratory infections in pediatric patients in Beijing. Methods Three hundred and nineteen specimens collected from pediatric patients with acute respiratory infections during Nov. 2003 to Feb. 2004 were selected to test this virus. These 319 specimens included 73 throst swabs from outpatient department and 246 nasopharyngeal aspirates form those hospitalized and had been determined as being negative far common respiratory viruses by virus isolation and/or indirect immunofluorescent assay. Polymerase chain reaction （PCR ） was performed to detect HBoV from these specimens using primers designed from the published sequences of NP1 gene from HBoV. The PCR products with expected molecular mass were selected randomly for sequencing and then sequences from thoas PCR products were compared with the sequences in the GenBank. Resuits Among theae 319 specimens detected, 13（4.1%） showed expected PCR products, suggesting they were positive for HBoV. The highest positive rate was among specimens collected from petients with bronchiolitis （10.9%, 5/ 46）, followed by petients diagnesed as bronchitis （6.3%, 2/32）. Age distribution of the children positive for HBoV was from 5 months to 5 years, especially thoas younger than 1 year of age. The positive rate for HBoV detoction was 50% among thoas aged from 6 months to 7 months（2/4） and 25%（2/8） among those aged from 9 months to 10 months. Neither of 28 children older than 5 years nor 103 infants younger than 5 months was positive for HBoV. Eive out of 13 amplicons were randomly selected for sequence analysis. When competed with the sequences of the prototype strains st1 and st2, these 5 amplicons shared high nucleotide sequence homology with st1, st2 （99.2% to 99.4% ） and among themselves （99.7% to 100%）. Conclusion These data suggest that some of acute respiratory infections in pediatric petients in Beijing are related to human bocavirus, which is most likely the second known parvovirus species being pathogenic to humans. Infants and young children aged from 5 months to 5 years, especially thoas younger than 1 year, are more likely to he infected by HBoV.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第5期385-388,共4页
Chinese Journal of Microbiology and Immunology
