摘要
目的 了解新近从北京地区发现的人类偏肺病毒 (humanmetapneumovirus ,HMPV)N蛋白编码基因的特征。方法 从经RT PCR检测HMPV阳性的临床鼻咽洗液标本中进行HMPVN蛋白全基因扩增、克隆至pUCm T载体中并进行测序 ,与GenBank中的多株HMPVN蛋白基因序列进行比较和进化树分析。结果 经扩增并测序 ,标本BJ1816和BJ1887N蛋白基因全长为 1185bp ,编码 394个氨基酸。BJ1816N蛋白基因与国际上第一株HMPV分离株NDL0 0 1和GenBank中其它的多株HMPV的核苷酸和氨基酸同源性分别为 86 .2 %～ 99.0 %和 96 .2 %～ 99.7%。BJ1887N蛋白基因与以上进行比较的各株HMPV的核苷酸和氨基酸同源性分别为 86 .6 %～ 97.0 %和 96 .4 %～ 99.5 %。BJ1816和BJ1887之间N基因的核苷酸和氨基酸同源性分别为 86 .6 %和 96 .4 %。提示BJ1816和BJ1887分属于HMPV的两个不同的基因进化簇。结论 从基因水平进一步证明新近发现的婴幼儿肺炎的新病原确为HMPV ,提示北京地区婴幼儿中同时存在两个不同进化簇 (即基因型 )的HMPV感染。
Objective A new respiratory virus, human metapneumovirus (HMPV) was recently identified from nasopharyngeal aspirates collected from infants and young children with acute respiratory infections in Beijing. To understand the characteristic of the newly discovered HMPV in Beijing, the N genes from two HMPVs which were in two different gene clusters from previous investigation were analyzed. Methods Full length of N genes from HMPV were amplified from two specimens, BJ1816 and BJ1887 collected from children hospitalized for acute respiratory infections in November 2002 by reverse-transcriptase and polymerase chain reaction (RT-PCR). The two PCR amplicons were sequenced after cloning into pUCm-T and the sequences were compared with the N genes from HMPVs in GenBank. Result The N genes amplified from specimens BJ1816 and BJ1887 were 1185bp in length. The deduced N proteins were 394 amino acids in length. The nucleotide identities of BJ1816 and BJ1887 compared with those from the first reported strain by Van den Hoogen (strain HMPV00-1) and those from Canada were 86.2%-99.0% and 86.6%-97.0%, respectively. The deduced amino acid similarities were 96.2%- 99.7% and 96.4%-99.5%. Between BJ1816 and BJ1887, the homology of nucleotides and that of amino acids were 86.6% and 96.4% respectively. Phylogenetic analysis showed that BJ1816 and BJ1887 fell into two distinct genetic clusters. Conclusion The sequence analysis of the complete N genes from two samples indicates that the pathogen associated with acute respiratory infection recently described in Beijing is HMPV. HMPVs of different genotypes may co-circulate in infants and young children in Beijing.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2004年第2期81-84,共4页
Chinese Journal of Microbiology and Immunology
