摘要
目的观察P物质(SP)对人增生性瘢痕(HS)组胺释放的影响及两者相互作用的条件。方法人HS和正常皮肤(NS)的标本分别取自笔者单位行整形术的8例烧伤患者,切下后立即用等渗盐水清洗,并修剪成0．5～1．0 mm^3的组织块。利用荧光分光光度计测定在不同浓度SP、不同SP作用时间、不同浓度Ca^(2+)的作用条件下,组织块中肥大细胞(MC)脱颗粒释放组胺的情况,并计算释放率。结果当SP浓度达到1×10^(-6)mol/L时,HS即有明显的组胺释放,释放率为(50．0±3．6)%,明显高于0 mol/L SP[(44．0±3．2)%,P＜0．01],并呈剂量依赖性。5×10^(-5)mol/L SP作用15 min内,HS已有约90%的组胺释放,并呈时间依赖性。当Ca^(2+)浓度为5×10^(-3)mol/L时,HS组胺释放率最高,基本呈剂量依赖性,但在Ca^(2+)浓度为1×10^(-3)mol/L时,释放率却出现一过性下降。在上述3个条件下,HS中SP对MC脱颗粒释放组胺的作用均明显强于NS(P＜0．01)。结论人HS中SP对MC的作用明显强于人NS,可能与瘢痕的增生和瘙痒有密切的关联。
Objective To investigate the influence of substance P（SP） on the release of histamine in the human hypertrophic scar tissue, and to explore the prerequisite of their interaction. Methods Tissue specimens of normal skin and hypertrophic scar from eight hospitalized patients were excised and cut into 0.5 to 1.0 mm^3 pieces, and the histamine release by mast cell（MC） under the stimulation of different concentration of SP and calcium, as well as the different affect time of SP, were determined with fluorescence spectrometer. Then the histamine release rate was calculated. Results There was obvious release of histamine when SP concentration was 1 ×10^-6mol/L , and the release rate was （50.0 ＋3.6）% , which was significantly higher than that by SP in the concentration of 0 mol/L[ （44.0 ＋ 3.2 ） % , P 〈 0.01 ]. Therefore it seemed to be dose-dependent. About 90% of histamine was released within 15 minutes of 5 ×10^-5 mol/L Substance P stimulation, and it was also time-dependent. The histamine release reached the peak when calcium concentration was 5 ×10^-3 mol/L, which seemed to be dose-dependent, but it decreased transiently when calcium concentration was 1 ×10^-3mol/L. In all occasions, the influence of SP on the histamine release by MC in hypertrophic scar（HS） was markedly higher than that in normal skin（NS） （ P 〈0.01 ）. Conelusion The influence of SP on the histamine release by MC in HS was markedly higher than that in NS, and it might be closely related to itching sensation and the formation of hypertrophic scar.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2006年第3期192-194,共3页
Chinese Journal of Burns
关键词
P物质
组胺
增生性瘢痕
Substance P
Histamine
Hypertrophic scar
