摘要
目的研究A型肉毒素(BTA)对皮肤神经末梢释放的神经肽SP、CGRP的影响及其对皮肤创伤愈合的意义。方法40只SD大鼠随机分为C组(空白对照组)和E组(肉毒素注射组),20只/组。肉毒素A预注射后7d,在鼠背部建立以4个注射点为中心的创面模型,面积1cm×1cm。测量术后3d和7d的创面面积。实时定量PCR(Real-timePCR)检测BTA注射后第7天(术前)和创面愈合后(术后14d)的皮肤组织中感觉神经肽P物质(SP)、降钙素基因相关肽(CGRP)以及创伤愈合中的重要分子TGF-β1和α-SMA的mRNA表达。结合免疫组化和图像分析技术检测切片中上述4种物质阳性染色的积分光密度OD值。结果①各组创面于术后14d基本愈合。与C组相比,E组术后3d、7d的创面面积无差异(P>0.05);②E组术前和术后14d的皮肤组织中SP、CGRP、TGF-β1和α-SMA的mRNA表达和OD值较C组明显降低,差异有统计学意义(P<0.05)。结论①局部皮下注射BTA不影响创面愈合的时间和速度;②BTA能减少创面愈合过程中皮肤神经末梢对SP、CGRP的释放和创面愈合过程中TGF-β1和α-SMA的表达。
Objective To investigate the effect of botulinum toxin type A on the expression of substance P and calcitionin gene-related peptide in wound healing. Methods Forty rats were randomly divided into Group C （Control） and Group E （Experimental）, 20 rats in each group. The wound-healing model was established by excised four full-thickness skin （1 cm × 1 cm, circled the injection site） in the back of all SD rats 7 days after BTA injection. The area of wound was measured and calculated at the 3^rd and 7^th day after skin lesion by computerized image analysis. On 0 and 14 days after operation, the content of substance P, calcitionin gene-related peptide, TGF-β1 and α-SMA were assayed by real-time polymerase chain reaction technique. The immunohistochemical staining and computerized image analysis were used to detect the OD value of positive staining of four substances. Results The wounds in all groups healed at 14 days after operation and the area in Group E had no significant difference with that of Group C at 3 days and 7 days after operation. The positive immuno-staining of SP, CGRP, TGF-β1 and α-SMA in Group E were significantly weaker than those in Group C. Conclusion Botulinum toxin type A can decrease the expression of SP, CGRP, TGF-β1 and α-SMA in wound healing in dose-dependent manner without delaying the healing time.
出处
《组织工程与重建外科杂志》
2009年第4期202-204,228,共4页
Journal of Tissue Engineering and Reconstructive Surgery
基金
上海市科委资助项目(074119606)
关键词
肉毒素A
P物质降钙素基因相关肽
创面愈合
瘢痕
Botulinum toxin type A
Substance P
Calcitionin gene-related peptide
Wound healing
Scar
