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周期性张应变对椎间盘纤维环细胞内游离钙离子浓度的影响 被引量：5

Effect of cyclic tensile strain on intracellular calcium concentration in cells of fibrous ring of intervertebral disk
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摘要 目的探讨周期性张应变对培养的大鼠椎间盘纤维环细胞内Ca2+浓度([Ca2+])的影响及其可能机制。方法采用激光扫描共聚焦显微镜和F luo-3/AM荧光探针标记技术,测定周期性张应变对纤维环细胞内[Ca2+]的影响,并分析了EGTA、维拉帕米和氯化钆预处理对此作用的影响。结果应变为10%、频率为1 Hz的周期性张应变1 m in使细胞内[Ca2+]增加1.13倍;EGTA可以阻断此作用;而维拉帕米和氯化钆仅可部分阻断。结论周期性张应变使纤维环细胞内[Ca2+]升高,细胞外钙内流起主要作用,且有不同的钙离子通道参与。 Objective To study the influence of cyclic tensile strain （CTS） on intracellular calcium concentration in the cells of fibrous ring of intervertebral disk and the mechanism. Methods The cells of fibrous ring of intervertebral disk were divided into 6 groups according to different treatment： negative control group; Two CTS groups receiving 10% , 1 Hz CTS for 1 or 5 min; EGTA ＋ CTS group treated with 5 mmol/L EGTA for 5 min before one-minute CTS; Verapamil ＋ CTS group treated with 10 μmol/L verapamil for 15 min before one-minute CTS; Gadolinium chloride ＋ CTS group treated with 10 μmol/L gadolinium chloride for 15 min before one-minute CTS. Laser scanning confocal microscope and Fluo-3/AM were used to investigate the changes of intracellular calcium concentration when the cells responded to cyclic tensile strain. Results The cyclic tensile strain increased the intracellular calcium concentration. EGTA inhibited the increase of intracellular calcium, but verapamil and gadolinium chloride partially blocked the effects. Conclusion Cyclic tensile strain induces the increase of intracellular calcium concentration, in which the influx of extracellular calcium plays an important role. Different calcium channels are involved in the process.
作者 郭志良 周跃 成敏 李华壮 曹国永 张伟 李兴鑫
机构地区 第三军医大学新桥医院骨科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2006年第11期1212-1214,共3页 Journal of Third Military Medical University
关键词 周期性张应变 纤维环细胞 细胞内钙 激光扫描共聚焦显微镜 cyclic tensile strain fibrous ring cell intracellular calcium laser scanning confocal microscope
分类号 R323.4 [医药卫生—人体解剖和组织胚胎学] R329-33 [医药卫生—人体解剖和组织胚胎学]
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参考文献13

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同被引文献54

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引证文献5

二级引证文献22

第三军医大学学报
2006年 第11期

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