摘要
目的观察周期性张应变力（CTS）对体外培养不同年龄兔软骨细胞产生糖胺多糖（GAG）的影响。方法选取雄性新西兰大白兔9只，按照年龄分为幼年组（2月龄）、成年组（8月龄）及老年组（31月龄）（每组3只），无菌条件下取双膝关节，将各年龄组兔膝软骨细胞进行消化分离和体外培养。将每个年龄组兔原代软骨细胞分别培养于2个BioFlex6孔培养板上，随机分为CTS组和对照组（每组6个样本），同置于培养箱内，CTS组每天CTS（sin 10％，0．5Hz，6h／次）作用6h，对照组不予特殊处理。CTS组与对照组在首次作用后第12、24、36、48、60、72小时分别吸取培养细胞上清液，Alcianblue染色沉淀法测定上清液GAG含量，比较两组GAG分泌的变化。结果CTS组与对照组相比，各年龄组兔软骨细胞GAG增加量在不同时间点差异均有统计学意义（P〈0．05）。老年组在起始时，两组GAG的增加量与年轻组的增加量无明显差异，但从第48小时起老年组增加量开始低于年轻组，差异有统计学意义（P〈0．05）。结论CTS可以促进兔软骨细胞产生GAG，且CTS刺激后年轻细胞比老年细胞产生更多的GAG。
Objective To observe the effects of cyclic tensile strain （CTS） on in vitro expression of glycosaminoglycans （GAG） in rabbit chondrocytes of different ages. Methods Nine male New Zealand rabbits were grouped into juvenile （2 months）, adult （8 months）, and senior （31 months） groups. The bilateral knee joints were harvested using sterile technique from each rabbits. In each age group, rabbit articular chondrocytes were cultivated in vitro after randomization into a control group and a CTS group, with 6 specimens in each. In the next 3 days, CTS was applied （sin 10%, 0. 5 Hz, 6 h/d） in the CTS group for 6 hours per day while no CTS was applied in the control group. After the first CTS treatment, the supernatant of cell culture was collected every 12 hours from each specimen in both groups to assess the GAG levels by Alcian blue assay. Results Expressions of GAG showed significant increases in both control and CTS groups in each age group （ P 〈 0. 05） . Different age groups showed significant differences in the GAG secretion over different time points. Compared with the younger cells, the older ones showed the most significant difference in growth between the control and CTS groups at 12 hours, though the older cells produced less GAG than the younger ones in both groups at the end of the experiment （72 hours） . Conclusions CTS can stimulate rabbit chondrocytes of different ages to secrete more GAG, and younger cells tend to produce more GAG than the older ones.
出处
《中华创伤骨科杂志》
CAS
CSCD
2011年第2期155-159,共5页
Chinese Journal of Orthopaedic Trauma
基金
基金项目：国家重点基础研究发展计划（2009CB526514）
国家自然科学基金（30872616）
关键词
软骨细胞
应变
应力
糖胺多糖
兔
Chondrocytes
Strain
Stress
Glycosaminoglycans
Rabbits
