摘要
目的观测恶性胶质瘤微血管三维构筑的形态学特征。方法采用人脑间变性星形细胞瘤组织连续切片,进行免疫组织化学标记微血管内皮细胞,利用计算机辅助的图片处理和三维重建技术进行微血管构筑的三维重建。结果成功构建了星形胶质细胞瘤部分微血管的三维结构。胶质瘤微血管与正常脑微血管迥然不同,其分布、分枝和走行怪异,在三维构筑上有其自身的特点,不完全遵从由大到小的分级规律,而且某段可向外呈盲管状膨出,部分血管为两端均不通的盲管。结论恶性星形细胞肿瘤的微血管不仅丰富,而且具有形态结构的多样性和异质性。这一特点对于认识恶性肿瘤血管生成三维结构特性、构建数字化肿瘤微血管模型及其在抗血管生成治疗上可能有重要意义。
Objective To observe three-dimensional （3-D） morphological features of microvessel architecture of human anaplastic astrocytoma. Methods Serial sections of human anaplastic astrocytoma were used to label microvascular endothelial cells with immunohistochemistry using anti-human FⅧ-RAg antibody. The serial images were taken and processed with computer-assisted 3-D reconstruction. Results Partial 3-D structures of the microvessels in anaplastic astrocytoma were successfully reconstructed. The morphology of the vessels was strange in their distribution and branching, which were completely different from normal mierovessels of the brain. Some of the tumor vessels showed chaotic structure and some showed tubiform caecum, Conclusion Mierovessels in anaplastic astroeytoma appeared multiformity and heterogeneity as well as abundance. 3-D reconstruction of such features might be of significance in identifying their architecture, constructing digital tumor microvessel models and targeting the vessels in anti-angiogenesis therapy.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第11期1148-1150,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(30370552)~~
关键词
胶质瘤
血管生成
微血管构筑
三维重建
glioma
angiogenesis
microvascular architecture
3-D reconstruction
