摘要
研究利用激光共聚焦显微镜和流式细胞仪分别定性、定量检测CXCR4的内化,观察vMIP-II对细胞表面趋化因子受体CXCR4内化的影响,用荧光分光光度计连续动态检测vMIP-II对细胞内钙离子浓度的影响,以阐明vMIP-II抗HIV/AIDS的作用机制。结果表明100 ng/ml vMIP-II在给药后的最初30 min能使细胞表面受体CXCR4内化达到最大值,内化率约为75%,且内化到细胞内的CXCR4并不能再循环到细胞表面。vMIP-II能诱导瞬间的高钙离子内流及细胞内钙离子库中钙离子的释放,证明了vMIP-II能引起细胞表面受体CXCR4的内化,内化的受体不再循环到细胞表面。
In order to investigate the role of viral macrophage inflammatory protein-II（vMIP-II） on the internalization and rood ulation of the surface chemokine receptor CXCR4, laser scanning eonfocal microscopy and flow cytometry were used to determine the quality and quantity of CXCR4 internalization, and the characteristics of Ca^21 ion influx was detected by spectrofluorophotometer. It was found that vMIP-II could induce internalization of CXCR4 molecules with a maximal rate of 75%, moreo wer, the internalized CXCR4 molecules was not to be recycled to the cell surface. In addition, vMIP-II could also induce the in flux of Ca^21 ions and prompt its release from calcium reservoir.
出处
《现代免疫学》
CAS
CSCD
北大核心
2006年第3期213-216,共4页
Current Immunology
基金
国家863计划资助项目(2003AA219060)
