摘要
目的 初步探明KSHV编码的病毒巨噬细胞炎症蛋白vMIP-Ⅰ和vMIP-Ⅱ在新疆经典型Kaposi＇s sarcoma（KS）患者的肉瘤组织、血液、唾液和尿液中的表达情况.方法 对8例新疆经典KS患者的新鲜冰冻肉瘤组织、血液、唾液和尿液标本进行基因组DNA提取,应用PCR扩增各标本中的vMIP-Ⅰ和vMIP-Ⅱ基因.结果 8例新疆经典型KS患者的肉瘤组织、血液、唾液和尿液中均获得较好的基因组DNA;同时8例组织标本全部扩增出vMIP-Ⅰ、vMIP-Ⅱ基因,但是8例患者的血液、唾液和尿液标本都未出现vMIP-Ⅰ和vMIP-Ⅱ基因表达.结论 新疆经典型KS患者肉瘤组织中均检测到vMIP-Ⅰ和vMIP-Ⅱ基因片段的表达,为深入研究卡波氏肉瘤的血管形成机制提供初步的依据.
Objective To observe the expression in sarcoma tissue,blood,salivaand urine of KSHV-encoded virus macrophage inflammatory protein vMIP-Ⅰ,vMIP-Ⅱ.Methods Genomic DNA was extracted from samples in 8 classic Kapsi＇ s sarcoma patients of Xinjiang,vMIP-Ⅰ and vMIP-Ⅱ gene were amplified by PCR.Results Genomic DNA were detected from sarcoma tissue,blood,saliva and urine of 8 classic Kapsi＇ s sarcoma patients,while vMIP-Ⅰ and vMIP-Ⅱ were amplified from all tissue samples,but the expression of vMIP-Ⅰ and vMIP-Ⅱ in blood,saliva and urine was negtive.Conclusions There is special expression of vMIP-Ⅰ and vMIP-Ⅱ in Kapsi＇ s sarcoma tissue.It provides a preliminary basis for further study of the mechanism of angiogenesis Kaposi＇s sarcoma.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第5期421-424,共4页
Chinese Journal of Experimental and Clinical Virology
基金
中国科学院上海巴斯德研究所/新疆医学大学第一附属医院联合免疫实验室种子基金（GRMY-2011-02）
