摘要
目的:探讨SDF-1α对乳腺癌细胞CerbB-2表达的影响。方法:SDF-1α与乳腺癌细胞MCF-7共培养,Western印迹法检测CerbB-2的表达差异;裸鼠成瘤实验联合免疫组织化学法检测SDF-1α在体内对乳腺癌细胞CerbB-2表达的影响;细胞迁徙及失巢凋亡实验对比分析SDF-1α对乳腺癌MCF-7细胞体外迁徙及抗失巢凋亡能力的影响。结果:Western印迹及裸鼠成瘤免疫组织化学检测结果显示,在体内体外SDF-1均能显著提高CerbB-2的表达,3组经不同浓度SDF-1α刺激的MCF-7细胞CerbB-2蛋白表达量均较空白对照组明显增高,其中400ng/mL组升高最为明显。侵袭小室实验发现,SDF-1α可明显增强乳腺癌MCF-7细胞株的细胞变形迁徙和侵袭破坏能力,培养6h后迁徙进入微孔膜的细胞数比阴性对照组显著增多[(172.27±9.72)vs(128.63±13.72),P<0.01];SDF-1α+MCF-7细胞发挥破坏Matrigel基质胶作用,进入微孔膜内的细胞数也高于MCF-7细胞[(52.28±9.24)vs(17.21±2.89),P<0.01]。悬浮培养的SDF-1α+MCF-7细胞比MCF-7细胞更容易聚集,形成相对致密的细胞团;200、400、800ng/mLSDF-1α作用24h后,MCF-7细胞的凋亡率分别为(9.72±1.11)%、(8.64±1.22)%和(10.36±1.31)%,与阴性对照组(18.41±1.71)%的差异有统计学意义(P<0.01)。结论:SDF-1α可显著提高乳腺癌细胞MCF-7的CerbB-2表达,拓宽了靶向药物Herceptin的应用范围。
Objective：To investigate the effect of SDF-1 α on the expression of CerbB-2 on the surface of human breast carcinoma MCF-7 cells. Methods：SDF-1α was co-cultured with human breast carcinoma MCF-7 cells. Expression of CerbB-2 in MCF-7 cells was detected by Western blotting in vitro. Nude mice tumorigenesis test and immunohistochemistry （ IHC ） were used to observe the effect of SDF-1α on the expression of CerbB-2 in vivo. The metastatic ability of MCF-7 cells was analyzed by Transwell migration test. The apoptosis of MCF-7 cells was detected by anoikis-resistant apoptosis assay. Results：Western blotting,nude mice tumorigenesis test and IHC showed that expression of CerbB-2 protein was significantly upregulated in human breast carcinoma MCF-7-cells after SDF-1α stimulation at three different concentrations. The effect was the strongest at SDF-1α 400 ng/mL. Transwell chamber test demonstrated that the migration and invation capabilities of MCF-7 cells were enhanced by co-culture with SDF-1α. After co-culture for 6 h, the cells migrating into micro-membrane were greatly increased compared with negative control [ （ 172.27 ±9.72） vs （ 128.63 ± 13.72） , P 〈0.01]. The MCF-7 cells exerted damaging effects on Matrigel after co-euhure with SDF-1α. The cells invading Matrigel were markedly elevated [ （ 52.28 ± 9.24） vs （ 17.21± 2.89）, P 〈 0.01 ]. Suspended MCF-7 ceils co-cultured with SDF-1α tended to accumulate thus forming relatively dense cell clusters. Apoptosis index （AI） of MCF-7 cells was （9.72 ±1.11 ） % , （ 8.64 ± 1.22） % , and （ 10.36 ±1.31 ） % after coulture with SDF-1α at 200,400, and 800 ng/mL compared with control group （ 18.41% ± 1.71% ）. Conclusion： SDF-1α significantly elevates the expression of CerbB-2 on the surface of breast carcinoma MCF-7 cells and widens the application area of targeting drug Herceptin.
出处
《肿瘤》
CAS
CSCD
北大核心
2009年第4期329-333,共5页
Tumor
