摘要
目的:探讨脂质体转染与电穿孔转染的效率,观察电转染对K562细胞凋亡的影响。方法:以GFP为报告基因,通过脂质体转染及电穿孔两种方式转染K562细胞,荧光显微镜下观察并计算转染效率,Annexin V和PI观察细胞凋亡率。结果:电转染较脂质体转染K562细胞的效率高,电穿孔转染效率10%左右,脂质体转染效率小于1%。电穿孔时K562细胞凋亡率为40%。结论:电转染较脂质体转染K562细胞的效率高,但细胞凋亡率也高。
Objective： To obtain higher efficiency in gene transfection into K562 cells and to study the effect of electroporation on cell apoptosis. Methods： Transfection efficiencies with different methods, electroporation and lipofectamine2000, were calculated under fluorescent microscopy using GFP （Green Fluorescent Protein） as a reporter gene. The frequency of K562 apoptosis was studied with Annexin V and PI. Results： The transfection efficiencies of electroporation（ 10% ）were higher than lipofectamine 2000 （ 〈 1% ）. The apoptosis frequency of the electroporation transfected K562 cells was 40%. Conclusion： The electroporation is a an ideal method for introduction of foreign gene into the K562 cells. But the electroporation induces cell apoptosis.
出处
《军医进修学院学报》
CAS
北大核心
2008年第5期420-421,共2页
Academic Journal of Pla Postgraduate Medical School
基金
国家基础研究973项目(2005CB522400)
国家自然科学基金(30572108)
关键词
脂质体
转染
电穿孔
细胞凋亡
liposomes
transfection
electroporation
apoptosis
