摘要
目的比较Lipofectamine2000、lipoplexes和apoE-lipoplexes三种脂质体对Li-7肝癌细胞的转染效率。方法构建普通阳离子脂质体lipoplexes和载脂蛋白apoE修饰脂质体apoE-lipoplexes;使用Lipofectamine2000,lipoplexes和apoE-lipoplexes三种脂质体运载以EGFP为报告基因的pGenesil-1质粒转染Li-7肝癌细胞,通过荧光显微镜观察EGFP蛋白的表达,流式细胞术检测三种脂质体的转染效率。结果 Lipofectamine2000、lipoplexes和apoE-lipoplexes三种脂质体转染效率分别为(18.63±0.57)%,(24.07±0.91)%,(28.93±0.50)%,3组间两两比较均有显著性差异(P<0.01)。结论 Li-7肝癌细胞的转染效率与脂质体的结构有关,提示脂质体结构改造可能成为提高转染效率的新策略。
Objective To compare the transfection efficiency of the three kinds of liposomes, Lipofectamine2000, lipoplexes and apoE-lipoplexes on Li-7 hepatoma cells. Methods To construct two kinds of gene vectors, one was normal cationic liposome （lipoplexes）, another was composed of liposomes （lipoplexes） and apolipoproteinE（apoE-lipoplexes）. The plasmid pGenesil-1 containing EGFP reporter gene was transfected into Li-7 cells using Lipofectamine2000, lipoplexes and apoE-lipoplexes, respectively. Then the expression of EGFP was observed by fluorescence microscopy, and the transfection efficiency of the three kinds of liposomes was measured by flow cytometry. Results Transfection efficiency of Lipofectamine2000, lipoplexes and apoE-lipoplexes was （18.63±0.57）%, （24.07±0.91）%and （28.93±0.50）%separately with the difference being significant among them （P〈0.01）. Conclusion Transfection efficiency of the liposome on Li-7 hepatoma cells is related to its structures, indicating that the structure transformation would be a new strategy for improving the transfection efficiency.
出处
《中华临床医师杂志（电子版）》
CAS
2014年第20期32-34,共3页
Chinese Journal of Clinicians(Electronic Edition)
基金
国家自然科学基金资助项目(81172136
30901821)
山西省高等学校创新人才支持计划资助(2011)
