摘要
运用盐酸控制解聚的方法,通过单因素考察和正交试验优化,得到了平均相对分子质量约为10 000的低分子螺旋藻多糖(LNPSP),用硫酸化试剂氯磺酸-吡啶合成3种不同取代度的LNPSP硫酸酯(SLNP-SP1,SLNPSP2,SLNPSP3),采用体外抗肿瘤实验测定3种硫酸酯化物对人肝癌细胞SMMC7721的活性。结果显示:低分子螺旋藻多糖的硫酸酯化物具有良好的体外抗肿瘤活性,与未修饰的LNPSP相比有显著性差异,且随着硫酸化程度的增高抗肿瘤活性也相应提高。
Low molecular weight polysaccharide of Spirulina Platensis （LNPSP） was obtained by hydrochloric acid degradation, and its average relative molecular mass was 10ku. The SLNPSP1, SLNPSP2, and SLNPSP3 of sulfated polysaccharides were synthesized by chlorosulfonic pyridine method, and their sulfur contents increased gradually. Their activities were determined through anti-tumor study in vitro on SMMC 7721. The results indicated that there was a very significant difference between the activities of SLNPSP and those of LNPSP, and the anti-tumor activity was increased with the sulfur content.
出处
《药物生物技术》
CAS
CSCD
2006年第2期119-122,共4页
Pharmaceutical Biotechnology
关键词
螺旋藻
多糖硫酸酯
降解
合成
体外抗肿瘤
Spirulina Platensis, Polysaceharide Sulfate, Degradation, Synthesization, Anti-tumor in vitro
