摘要
目的:研究西罗莫司酯化物(Temsirolimus)对肾癌细胞786-0增殖与凋亡的影响,并探讨分子作用机制.方法:采用显微镜形态学观察和MTT法检测细胞增殖抑制;流式细胞仪分析用药48h后肾癌细胞周期变化和细胞凋亡率;Western免疫印迹法检测P70S6K,4EBP-1蛋白水平表达变化.结果:西罗莫司酯化物作用786-0细胞的IC50=3.31μmol/L;给药48h后,细胞明显阻滞于G0/G1期,G2/M期细胞比例减少,细胞凋亡率增加;肾癌786-0细胞中P70S6K和4EBP-1蛋白的磷酸化水平明显下降.结论:西罗莫司酯化物能显著抑制肾癌细胞786-0的细胞增殖作用,阻滞细胞周期于G0/G1期且诱导细胞凋亡,其机制可能与P70S6K和4EBP-1磷酸化相关.
Aim： To investigate anti-tumor effects and its mechanism of Temsirolimus on 786 0 renal carcinoma cells. Method： MTT colorimetry method, fluoroscope, FCM combine PI and Annexin V FITC double pigmenta tion method and Western blot method were used. Results： The cell proliferation w ere obvious suppressed after Ireated with different concentration Temsirolimus for/18 hours, and IC50 is 3.31 μmol/L. The apoplo sis induced by Temsirolimus of 786 0 cell is concentration dependent with Tcmsirolimus increased from 0.01μmol/L. to 10μmol/L., and theccll showedG0/G1 phase block. Observed with western blot method, the expression ofP70S6K and4EBP 1 protein were decreased obviously. Conclusion： Temsirolimus can in terfere cell growth cycle of 786 0 renal carcinoma cell and suppress cell growth. The suppression effect is concenlration dependent. The effect depends not only from the nonspecific cytotoxic but also from induced cell apoptosis. The possible mechanism is related to P70S6K and 4EBP-1 pathway.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第2期1-6,共6页
Journal of Southwest University(Natural Science Edition)
基金
国家新药创制重大专项课题(2010ZX09401-306-4-4)
重庆市科委重点攻关项目(2011GGC10006-33)
