摘要
目的:研究免疫增强活性较好的酵母葡聚糖硫酸酯(SPS)的修饰条件。方法:通过L9(3)4正交设计对氯磺酸-吡啶法制备SPS的工艺进行优化,并以MTT法测定其对小鼠脾淋巴细胞体外增殖的促进作用。结果:最佳修饰条件为氯磺酸∶吡啶=1∶6(V/V),反应温度70℃,反应时间3h,硫酸基取代度可达1.092。在50～200μg.mL-1内,最佳工艺所得SPS对小鼠脾淋巴细胞生长有显著促进作用,平均增殖率可达89.85%。结论:优化方法制备的SPS对小鼠脾淋巴细胞生长具有显著的促进作用。
Objective： To optimize the modification conditions for the preparation of sulfated glucan from Saccharomyces cervisiae（SPS） with high immune enhancement activity.Methods： SPS was prepared using chlorosulfonic acid-pyridine method by L9（3）4 orthogonal design,immunomodulatory activity of SPS was examined by the lymphocytes experiment.Results： The optimized conditions were volume ratio of chlorosulfonic acid to pyridine at 1：6,reaction temperature at 70℃ and reaction time for 3 h.The content of sulfur in SPS and the degree of substitution（Ds） were achieved to be 12.78% and 1.09.In the range of 50～200 μg·mL-1,the lymphocytes-activating activity was significantly enhanced by SPS compared with control group（P0.01）,with the mean proliferation rate at 89.85%.Conclusion： The SPS prepared by the proposed method has higher sulfur content and enhances the proliferation rate of lymphocytes from the spleen of ICR mice significantly
出处
《药学与临床研究》
2011年第3期224-226,共3页
Pharmaceutical and Clinical Research
关键词
葡聚糖
酵母
氯磺酸-吡啶法
淋巴细胞
Glucan
Saccharomyces cervisiae
Chlorosulfonic acid-pyridine
Lymphocytes
