摘要
目的:探讨壳聚糖(chitosan)包裹DNA疫苗皮肤免疫效果。方法:以chitosan包裹不同的草原兔尾鼠卵透明带3(LZP3)基因DNA疫苗pcDNA3-Lzp3(pLzp3)和pLC,通过皮肤擦拭免疫小鼠,以ELISA方法检测抗体水平,对卵巢做病理切片检测。结果:ELISA结果显示用chitosan包裹的pLC组在皮肤免疫中显示出优势,卵巢病理切片结果正常。结论:Chitosan包裹质粒皮肤免疫小鼠较纯质粒免疫效果更为显著。
Objective： To investigate the immune response of topical immunization of new choitosan nanoparticles coating DNA vaccine. Methods： Differem DNA vaccines of Lagurus logurus zona peUucida 3 gene （Lzp3）, pcDNA3 - Lzp3（pLzp3）and pLC coated with chitosan immunized NIH mice by topical immtmizafion. The antibodies were measured by ELISA. Histology was performed on representative 8 -μm secfiom of ovaries from immunized mice. Results： ELISA showed that the effect of the pLC coated with the chitosan was the best. Histology analysis indicated that no disruption of follicular development. Conclusion： This topical immunization using the mixture of the chitosan - DNA in comparison with the naked DNA was the more effective immtmizafion technique.
出处
《生物技术》
CAS
CSCD
2006年第2期68-71,共4页
Biotechnology
基金
国家自然科学基金资助项目(No.30360062)
