摘要
目的:探讨金属硫蛋白在食管鳞癌中的表达及其与癌细胞增殖、凋亡的关系。方法:采用免疫组化法检测92例食管鳞癌组织中的金属硫蛋白(MT)、增殖细胞核相关抗原(Ki-67抗原)的表达,并用TUNEL法检测细胞凋亡指数。结果:92例食管鳞癌标本中65例MT呈阳性表达,阳性率为70.6%,明显高于正常食管粘膜上皮组织(3/20,P<0.01);MT表达与食管鳞癌患者年龄、性别、浸润深度及病变长度无关,而与组织分化程度、TNM分期、淋巴结转移密切相关,MT与Ki-67抗原表达强度呈正相关,Pearson列联系数=0.227(P<0.05)。随MT表达强度的增加,癌组织凋亡指数逐渐降低。结论:MT表达与食管鳞癌的发生、发展密切相关,MT能促进癌细胞增殖,抑制细胞凋亡,可作为食管鳞癌治疗的新靶点。
Objective： To study the expression of metallothionein（MT） in esophagus squamous carcinoma and its relationship with cell proliferation and apoptosis. Method： Immunohistochemistry（S-P） was used to examine the expression of MT and Ki-67 in 92 cases of esophagus squamous carcinoma, and TUNEL （terminal deoxynucleoti- dyl transferase mediated UTP nick end labeling） was used to examine the ceil apoptosis index. Result： The level of MT expression in esophagus squamous carcinoma（70.6% ,65/92） was obviously higher than that in normal mucous membrane of esophagus（15.0% ,3/20）. The expression of MT had no relation with the age, gender, length of lesion, depth of infiltrate, but had close relation with the lymph node metastasis, degree of differentiation, and PTNM stage. There was a positive correlation between MT expression and Ki-67 antigen expression （Pearson＇sR = 0. 227, P 〈 0.05）. Following the increasing of expression degree of MT, the apoptosis index of cancerous ceil was degraded. Conclusion： MT is highly expression in esophagus squamous carcinoma, which is closly related with esophagus squamous carcino-genesis and development. MT can promote carcinoma cell proliferation, and inhibit cell apoptosis, suggesting it is a new target for treating esophagus squamous carcinoma.
出处
《山东大学学报（医学版）》
CAS
北大核心
2006年第4期384-388,共5页
Journal of Shandong University:Health Sciences
