摘要
目的探讨小鼠烫伤后肠黏膜相关淋巴细胞变化与肠道细菌移位的关系。方法将40只BALB/c小鼠随机分为正常对照组及烫伤后12、24、72 h组,每组10只。正常对照组不致伤, 其余各组小鼠背部造成20％TBSAⅢ度烫伤后,按时相点处死并留取标本。计数全段小肠集合淋巴结 (PP结)个数及淋巴细胞总数。应用流式细胞仪检测小鼠PP结CD3+、CD4+、CD19+淋巴细胞比例和绝对数,并检测主要脏器肠道细菌移位率。结果烫伤后12、24、72 h组PP结淋巴细胞总数分别为 (4．05±0．28)×106、(2．64±0．39)×106、(2．83±0．46)×106个,均少于正常对照组的(4．54±0．58)× 106个(P<0．05或0．01)。与正常对照组比较,烫伤后72 h组PP结淋巴细胞悬液中CD3+、CD4+百分比明显降低(P<0．05)。伤后各组小鼠CD3+、CD4+、CD19+淋巴细胞绝对数明显减少。各烫伤组肠道细菌移位率分别为16％、52％、30％,均高于正常对照组(4％),其中烫伤后24、72 h组与正常对照组比较,差异有统计学意义(P<0．05)。结论烫伤后肠黏膜相关淋巴细胞减少是肠源性感染的重要因素。
Objective To investigate the relationship between the changes in enteral mucosal lymphocytes and bacterial translocation in severely scalded mice. Methods Forty Balb/e mice were randomly divided into 4 groups, with 10 in each group, i.e. normal control, 12-postburn-hour group（12PBH） ,24PBH and 72PBH groups. The mice in the latter 3 groups were inflicted with 20% TBSA full-thickness scald on the back, and were sacrificed at respective time point. The number of Peyer＇s patches of entire small intestine and the number of lymphocytes in Peyer＇s patches were counted. The percentage and the number of CD3^＋ ,CD4^＋ and CD19^＋ lymphocyte subpopulations in Peyer＇s patches were determined by flow cytometry. The incidence of enteral bacterial translocation was determined by routine methods. Results The number of total lymphocyte in viable Peyer＇s patches was （4.05 ±0.28）×10^6,（2.64 ±0.39）×10^6 and （2.83 ±0.46）×10^6 in 12PBH, 24PBH and 72PBH groups, respectively, which was significantly less than that in control group（4.54 ±0.58） × 10^6（ P 〈0.05 or 0.01 ）. The absolute number of CD3^＋ ,CD4^＋ and C D19^＋ lymphocyte subpopulations of Peyer＇s patches in PBH groups was markedly decreased when compared with that in normal control group. The incidence of bacterial translocation was 4% in normal control group, and they were lower than that in 12PBH （16%）, 24PBH（52% ） and 72PBH（30% ） groups, and the difference of the incidence between normal control group and 24 and 72PBH groups was significant （ P 〈 0.05）. Conclusion The results indicate that the number of enteral mucosal lymphocyte plays important role in enteral bacterial transloeation in severely scalded mice.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2006年第2期117-120,共4页
Chinese Journal of Burns
关键词
烧伤
小肠
淋巴细胞
细菌移位
肠集合淋巴结
Burns
Intestine,small
Lymphoeytes
Bacterial transloeation
Peyer＇s patches
