摘要
背景和目的已有的研究表明人类白细胞抗原(HLA)在抗原呈递及T细胞识别抗原的过程中起关键作用,此外还与肿瘤细胞的免疫杀伤及免疫逃避有着密切的关系。本研究探讨了人肺腺癌细胞系A549、Calu6中HLA-A、HLA-B、HLA-DR等位基因的存在状况。方法分离A549、Calu6细胞DNA,分别行PCRSSP法扩增、电泳后紫外透射扫描,根据反应格局表对HLA-A、HLA-B、HLA-DR进行判定。结果A549与Calu6细胞中HLAA、HLAB基因较杂合子均有缺失,而HLA-DR基因无缺失。A549细胞HLAABDR的基因分型为HLA-A30、HLA-B44、HLA-DR7/HLADR53。Calu6细胞HLAAB-DR的基因分型为HLAA01、HLAB08、HLADR17/HLADR52。结论肺腺癌中存在HLAⅠ和HLAⅡ基因。HLAⅠ基因可能在肿瘤细胞传代过程中发生选择性丢失,而HLA-DR基因完整保留。检测肿瘤HL-A对了解其免疫学行为及建立肿瘤特异性杀伤淋巴细胞(CTL)模型具有重要意义。
Background and objective It has been confirmed that human leucocyte antigen （HLA） may play very important roles in the process of antigen presenting and antigen distinguishing. HLA has a close relationship with the immunity killing and immunity escape in cancer. HLA-ABDR alleles were detected in A549 and Calu-6 lung cancer cell lines by PCR-sequence specific primers （PCR-SSP） in this research. Methods DNA of A549 and Calu-6 was purified and PCR-SSP was practiced. Then the gel was scanned in ultra-violet. HLA-A, HLA-B and HLA-DR were determined with special response list. Results HLA-A and HLA-B in A549 and Calu-6 were not integral, but HLA-DR was integral. The genotype of HLA-ABDR for A549 was： HLA-A30, HLA-B44, HLA-DR7/HLA-DR53. The genotype of HLA-ABDR for Calu-6 was： HLA-A01, HLA-B08, HLA-DR17/HLA-DR52. Conclusion Human adenocarcinoma cell line exists both HLA-Ⅰ and HLA-Ⅱ genotypes. Selective loss of HLA-Ⅰ gene might be occured during tumor generation, but all the HLA-Ⅱgenes remain. Detection of tumor HLA is necessary to the acknowledgment of tumor immunology behavior and foundation of tumor specific cytotoxic T lymphocyte.
出处
《中国肺癌杂志》
CAS
2006年第2期113-116,共4页
Chinese Journal of Lung Cancer
基金
国家自然科学基金(No.30400440)
第三军医大学博士创新基金资助~~
