摘要
目的：探讨ＨＬＡＡ０２型及其亚型与启东肝细胞癌的相关性。方法：用ＰＣＲＳＳＰ法分析启东肝细胞癌和正常人中ＨＬＡＡ０２型及其亚型的分布。结果：１０３例经病理诊断的肝细胞癌中５７例为ＨＬＡＡ０２型，其比例达到５５％，显著高于启东正常人群ＨＬＡＡ０２型３２％（３３／１０２）的比例，Ｐ＜０．００１。同时揭示包括ＨＬＡＡ０２型在内的ＨＬＡＡ位点的等位基因多为杂合型。启东肝细胞癌ＨＬＡＡ０２型中Ａ０２０１亚型所占的比例稍高于正常人群，但没有显著差异。分析比较表明，启东肝细胞癌中ＨＬＡＡ０２型的相对集中与当地肝癌中ｐ５３基因２４９密码子突变的高频度发生并没有关联。结论：在乙肝病毒和黄曲霉毒素均为危险因子的启东地区，ＨＬＡＡ０２型与肝细胞癌密切相关。
Objective: The
association of HLAA02 genotype and its subtypes with hepatocellular carcinoma (HCC) was
investigated in a prevalent area, Qidong of China. Methods: The HLAA02 genotype were
studied by using PCRSSP. Results: 57 of 103 HCC were found to be of HLAA02 genotype. In
contrast, 33 of 102 normal people of Qidong were identified to be of HLAA02. The difference is
statistically highly significant ( P <0.001). The HLAA locus alleles were found to be mostly
heterozygous. The distribution of A02 subtypes was similar to that of normal peoples. 249
codon mutation status of p53 gene of HCC was identified by PCR and HaeIII RFLP analysis,
confirmed by DNA sequencing. The results showed that the 249 codon mutation frequency of
p53 gene was not associated with the A02 genotype. Conclusion: In an area where HBV and
aflatoxin were risk factors, a close association of HLAA02 genotype with hepatocellular
carcinoma was identified. This genotype clustering was not associated with the unique 249
codon hotspot mutation of the p53 gene.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第7期305-307,310,共4页
Chinese Journal of Immunology
