摘要
目的观察不同饲养期铁缺乏大鼠畸变产物耳声发射(DPOAE)的改变与耳蜗毛细胞改变的关系,探讨铁缺乏引起听毛细胞损伤的可能机制。方法以缺铁饮食饲养法复制缺铁Wistar大鼠模型33只,标准饮食喂养33只作对照。每组分别于喂养第4,8,12周各随机取11只测双耳DPOAE幅值,并处死做耳蜗扫描电镜。结果缺铁组第4周,36．4％耳数(8／22)出现DPOAE幅值中、高频率段下移,但扫描电镜下未发现毛细胞病理改变;缺铁至第8周,59．1％耳数(13／22)出现DPOAE幅值中、高频率段程度较重的下移,13耳中,3个耳蜗出现不同程度外毛细胞静纤毛散乱、扭曲、倒伏、融合等病理改变;缺铁至第12周,55．6％耳数(10／18)DPOAE幅值中、高频率段下移。10耳中,5个耳蜗出现不同程度外毛细胞静纤毛散乱、扭曲、倒伏、融合,缺失等病理改变,病变范围扩大。结论铁缺乏可使Wistar 大鼠DPOAE以及耳蜗毛细胞发生改变,DPOAE改变发生在毛细胞改变之前,DPOAE幅值下降到一定程度延缓或停止,但毛细胞病理改变仍有加重趋势。
Objective To obsreve DPOAE change in rats at different iron-deficient stages. Methods Sixty-six healthy Wistar rats were randomly divided into control group （ 33 rats） and iron-deficient group （ 33 rats）. The iron-deficient group were fed with iron-different diet. DPOAEs were measured and cochlear hair cell were examined by scanning electron microscopy at the 4th ,8th and 12th week in both groups. The iron-deficient diet was continued to the 8th week but stopped at the 12th week. Results A significant decrease of DPOAE threshold levers at 2 kHz,4 kHz in iron-deficient group was obserred at the 4th week, More frequencies were affected as the iron-deficient diet was continued to the 8th week, but stop at the 12th week. Conclusions Irondeficient can effect the function and structure of cochlear hair cells.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2006年第1期6-9,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
国家自然基金(30472124)
关键词
铁缺乏
WISTAR鼠
畸变产物耳声发射
毛细胞
Iron deficiency
Wistar rat
Distortion pmdnct otoacoustic emissions
Hair cell
