摘要
目的:研究Vp16对人肺腺癌A549细胞生长特性及Fas/FasL表达的影响。方法:用MTT法检测细胞增殖特性,FCM观察细胞周期及凋亡,RT-PCR测定mRNA表达的变化。结果:Vp16以时间和剂量依赖方式作用于A549细胞的增殖;不同浓度的Vp16作用A549细胞,使其阻断在不同的细胞周期,且均能明显下调FasL的mRNA的表达(P<0.05),而对FasmRNA的表达差异无显著性意义(P>0.05)。结论:Vp16直接作用A549细胞主要引起细胞坏死,Vp16可能通过下调FasL的mRNA的表达,防止肿瘤细胞免疫逃逸,该结果为临床合理联合用药提供了实验依据。
Objective：To investigate the effects of Vp16 on the growth characteristics and Fas/Fasl expression of A549 cells.Method：The growth rate, cell growth cycle and apoptosis were detected by MTr assay and FCM analysis respectively, The gene expression was determined by reverse transcription polymerase chain reaction （RT- PCR）. Result：Vp16 inhibited the cell proliferation more effectively as dose and time increased. The cell cycle was arrested in dose-dependent manner, and FasLmRNA expression was dramatically decreased （P〈0.05）, meanwhile Fas mRNA expression was not significantly different （P〉0.05） compared with the control. Conclusion：Vp16 cn mainly induce necrosis of human lung carcinoma A549 cells and prevent the immune escape of tumor cell by retarding FasLmRNA expression, which is valuable in selecting anti-cancer drugs in clinic therapy.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2006年第3期237-239,共3页
Chinese Journal of Rehabilitation Medicine
