摘要
目的明确外周血白细胞中结核分枝杆菌 DNA 检测在肺结核诊断中的意义。方法应用聚合酶链反应技术,对95例肺结核患者外周血标本、84例肺结核患者痰标本进行了结核分枝杆菌DNA 检测。结果外周血、痰标本中,结核分枝杆菌 DNA 阳性率分别为85%和57%;对44例患者外周血、痰标本配对同时检测总阳性率可达91%。结论外周血白细胞中结核分枝杆菌 DNA 检测用于肺结核的诊断,可提高肺结核诊断的敏感性和准确性,明显优于痰标本,可作为肺结核诊断较可靠的指标。
Objective To detect Mycobacterium tuberculosis in peripheral blood from patients with pulmonary tuberculosis.Methods Using polymerase chain reaction(PCR)technique,we amplified DNA of Mycobacterium tuberculosis in peripheral blood from 95 pulmonary tuberculosis patients.Re- sults The postivity rate of PCR in blood was 81/95(85%)which was significantly higher than that in sputum 48/84(57%).Combinative detection of blood and sputum in 44 patients showed that the sensi- tiveness and correctness of detection was increased,and the postivity rate was 91%.Conclusions This result indicated that the detection of DNA of Mycobacterium tuberculosis in peripheral blood was very useful for diagnosis of pulmonary tuberculosis.
关键词
肺结核
结核分枝杆菌
DNA
聚合酶链反应
Tuberculosis,pulmonary
Polymerase chain reaction
Mycobacterium tuberculosis
DNA,bacterial
