摘要
目的：明确外周血吞噬细胞内外伤寒沙门菌Ｖｉ基因检测在伤寒诊断中的意义。方法：高速离心沉淀、低渗溶解红细胞法分离８５例临床拟诊伤寒患者外周血吞噬细胞（内）及吞噬细胞外（血清中）伤寒沙门菌，巢式聚合酶链反应扩增伤寒沙门菌Ｖｉ基因；并与单管套式聚合酶链反应扩增粒细胞内伤寒沙门菌Ｈ基因、巢式聚合酶链反应扩增单核细胞内伤寒沙门菌Ｖｉ基因、１次聚合酶链反应扩增血清中伤寒沙门菌Ｈ基因比较。结果：巢式聚合酶链反应扩增吞噬细胞内外伤寒沙门菌Ｖｉ基因阳性率（６４．７％）不低于巢式聚合酶链反应扩增单个核细胞内伤寒沙门菌Ｖｉ基因（６０．０％）及单管套式聚合酶链反应扩增粒细胞内伤寒沙门菌Ｈ基因（５７．６％）（Ｐ＞０．０５），且高于１次聚合酶链反应扩增血清中伤寒沙门菌Ｈ基因（３２．９％）（Ｐ＜０．０１）。结论：巢式聚合酶链反应扩增外周血吞噬细胞内外伤寒沙门菌Ｖｉ基因，具有节俭、简便、灵敏、特异的优点，是实验室诊断伤寒的理想指标。
Objective:To clarify the significance of detecting Salmonella typhi Vi gene in peripheral blood serum and macrophages in diagnosis of typhoid fever.Methods:Detection of S.typhi Vi gene in peripheral blood and macrophages from 85 patients with clinical typhoid fever by nested PCR amplification method,S.typhi H gene in granulocytes by single tube nested PCR,S.typhi Vi gene in mononuleocytes by nested PCR and S.typhi H gene in serum by PCR.Results:There were no significant differences (P>0.05)in the positivity rate among S.typhi Vi gene in peripheral blood serum and macrophages(64.7%),S.typhi Vi gene in mononucleocytes(60.0%)and S.typhi H gene in granulocytes(57.6%);but the positivity rate of them was significantly higher(P<0.01)than that of S.typhi H gene in serum(32.9%).Conclusions:The nested PCR amplification technique is a simple,time saving,sensitive and specific method for detecting S.typhi Vi gene in peripheral blood serum and macrophages.It may be used as an ideal parameters for laboratory diagnosis of typhoid fever.
出处
《承德医学院学报》
1999年第2期108-110,共3页
Journal of Chengde Medical University
