摘要
目的:观察红景天属植物红景天的主要有效成分红景天苷对全脑缺血再灌注大鼠脑组织内白细胞介素1β含量的影响,探讨其对脑缺血再灌注损伤的保护作用。方法:实验于2004-04/2005-04在武装警察部队医学院中心实验室完成。①选用健康成年Wistar雄性大鼠24只。随机将大鼠分为3组:红景天苷组、模型组及假手术组,每组8只。红景天苷组:红景天苷[由华东理工大学生物反应器工程国家重点实验室生物分离研究室提供(生产批号:20040112,纯度>95%)]36mg/(kg·d)灌胃。模型组和假手术组:按给药组相同体积每日蒸馏水灌胃。各组连续灌胃7d后,用改良的Pulsinelli4-血管阻断法,复制大鼠急性全脑缺血30min再灌注60min损伤模型,造模成功后即刻断头取脑。②采用酶联免疫吸附法测定大鼠脑组织匀浆中白细胞介素1β含量。③计量资料差异比较采用LSD方差分析。结果:大鼠24只均进入结果分析。大鼠脑匀浆液中白细胞介素1β含量:模型组明显高于假手术组、红景天苷药物组[(85.00±24.05),(43.90±22.67),(45.1±17.02)ng/g,P<0.01]。红景天苷药物组与假手术组比较,差异不明显(P>0.05)。结论:红景天苷可抑制大鼠缺血再灌注所引起的白细胞介素1β表达的增加,对缺血再灌注损伤鼠脑具有保护作用。
AIM： To study the effect of salidroside on content of interleukin-1 beta （IL-1β） of rats with global cerebral ischemia-reperfusion injury, so as to investigate its protection on global cerebral ischemia-reperfusion injury.
METHODS： The experiment was completed in the Central Laboratory of Medical College of Chinese People＇s Armed Police Forces from April 2004 to May 2005. ① Totally 24 healthy adult male Wistar rats were were randomly divided into three group： salidroside group, model group and sham operation group with 8 in each group. Salidroside group： Rats were perfused with 36 mg/（kg·d） salidroside [provide by Biological Separation Laboratory, National Key Laboratory of Bioreactor of North China University of Technology, batch number： 20040112, purity 〉95%]. Rats in model group and sham operation group were perfused with the same volume of distilled water. After 7 days, acute injury models with 30-minute ischemia and 60-minute reperfusion were blocked with modified Pulsinelli- 4 vessel occlusion. Rats were killed to obtain their brains after successful modeling.②IL-1β content was assayed with enzyme-linked immunoadsordent assay （ELISA）.③Measurement data were compared with ISD analysis of variance.
RESULTS： Totally 24 rats entered the final analysis. IL-1β content in homogenate liquid： Content in model group was higher that that in sham operation group and salidroside group [（85.00±24.05）, （43.90±22.67）, （45.1±17.02） ng/g, P 〈 0.01]. There was not significant difference between salidroside group and sham operation group （P 〉 0.05）.
CONCLUSION： Salidroside can inhibit increase of IL-1β expression induced by ischemia-reperfusion injury and also can protect brain of rats induced by ischemia-reperfusion injury.
出处
《中国临床康复》
CSCD
北大核心
2006年第11期30-32,共3页
Chinese Journal of Clinical Rehabilitation
