摘要
背景:脑海马结构是与学习记忆有关的脑区,一般认为与空间认知活动密切相关。在脑缺血后发生的过氧化应激引发海马区和齿状回DNA损伤和DNA修复能力下降,其学习记忆功能也相应下调。目的:探讨红景天对全脑缺血再灌注大鼠学习记忆功能区域海马区及齿状回核酸表达的影响。设计:随机对照实验。单位:武警医学院中心实验室。对象:实验于2002-04/2004-04在武警医学院中心实验室完成,选用Wistar雄性大鼠60只,随机分为5组,每组12只。①红景天1.2g/(kg·d)剂量组。②红景天0.48g/(kg·d)剂量组。③川芎嗪80mg/(kg·d)对照组。④模型组:按给药组相同体积每日蒸馏水灌胃。⑤假手术组:按给药组相同体积每日蒸馏水灌胃。方法:各组大鼠连续灌胃给药7d,用改良的Pulsinelli4-血管阻断法,复制大鼠急性全脑缺血再灌注损伤模型。其中假手术组不灼烧椎动脉,不夹闭颈总动脉。模型制备后用吖啶橙染色法观察脑海马区和齿状回DNA、RNA的表达变化。主要观察指标:各组大鼠脑海马区及齿状回DNA、RNA的表达。结果:60只大鼠均进入结果分析。假手术组DNA和RNA分布均匀,荧光反射边界清晰,反应强度较强;模型组的DNA和RNA荧光反射边界不清,反应强度明显减弱;红景天1.2g/(kg·d)剂量组的DNA和RNA分布均匀,荧光反射边界清晰,反应强度明显增强,与假手术组及川芎嗪80mg/(kg·d)对照组表达分布基本相同。红景天0.48g/(kg·d)剂量组荧光反应强度及分布与模型组比较无明显改变。结论:手术组DNA和RNA荧光反射不清,可能与脑缺血再灌流损伤中氧化应激引起DNA链断裂有关。给予红景天1.2g/(kg·d)剂量大鼠脑海马区及齿状回DNA和RNA荧光反射边界清晰,表明红景天能够抑制氧化应激引起的DNA链断裂,对全脑缺血再灌注大鼠学习记忆功能区域海马区及齿状回具有保护作用。
BACKGROUND： Hippocampol formation of brain, a cerebral area related with learning and memory, is closely related to spatial cognitive activity. Peroxidative stress following the onset of cerebral ischemia can induce DNA injury in hippocampal ＇area and dentate gyms, the fall of the ability of DNA plerosis and correspondingly a deeliue in the function of learning and memory. OBJECTIVE： To investigate the effect of hongjingtian on the expression of nucleic acid of hippocampal area and dentate gyrus, the learning and memory area of rats with complete cerebral ischemia-reperfusion injury. DESIGN： Randomized controlled study. SETTING： Central Laboratory of Armed Police Medical College. PARTICIPANTS： The experiment was completed at the Central Laboratory of Armed Police Medical College frnm April 2002 to April 2004. Totally 60 Wistar male rats were selected and divided randomly into 5 groups with 12 in each group.①1.2 g/（kg·d） hongjingtian group; ② 0.48 g/（kg·d） hongjingtian group;③80 mg/（kg·d） ligustrazine control group; ④Model group： Rats were perfused daily with distilled water of a volume the same as that in medication group,⑤Sham-operation group： Rats were perfused daily with distilled water of a volume the same as that in medication group, METHODS： Rats in each group were perfused incessantly for 7 days, Modified Pulsinelli-4 vessel blocking method was used to reproduce the rat model of acute complete cerebral ischemia-reperfusion. Rats in sham-operation group were not treated with the operation of burning vertebral artery and clipping common carotid artery. The changes of DNA and RNA expressions in cerebral hippocampal area and dentate gyrus were observed with acridine orange staining method after model establishment, MAIN OUTCOME MEASURES： Expression of DNA and RNA in cerebral hippocampal area and dentate gyrus of rats in each group, RESULTS： All 60 rats entered the final analysis. DNA and RNA in shamoperation group distributed evenly, border of fluorescent reflex was legible and response intensity was strong, Border of DNA and RNA fluorescent reflex was illegible and response intensity was obviously weak in model group. DNA and RNA in 1.2 g/（kg·d） hongjingtian group distributed evenly, border of fluorescent reflex was legible and response intensity was strong. The distribution in 1.2 g/（kg·d） hongjingtian group was similar to that in sham-operation group and 80 mg/（kg·d） ligustrazine group, but fluorescent response intensity and distribution in 0.48 g/（kg·d） hongjingtian group was not of obvious difference as compared with that in model group. CONCLUSION： The illegibility of DNA and RNA fluorescent response in operation group is related with the breakage of DNA chain induced by oxidative stress during cerebral ischemia-reperfusion injury. Border of DNA and RNA fluorescent reflex in hippocampal area and dentate gyrus is legible in 1.2 g/（kg·d） hongjingtian group, suggesting that hongjingtian can inhibit the breakage of DNA chain induced by oxidative stress and protect learning and memory function in hippocampal area and dentate gyrus of rats with complete cerebral ischemia-reperfusion.
出处
《中国临床康复》
CSCD
北大核心
2005年第32期232-233,F0003,共3页
Chinese Journal of Clinical Rehabilitation
