摘要
目的:观察菟丝子水提物对活性氧(ROS)所致人精子顶体和超微结构的氧化损伤的保护作用,探讨菟丝子治疗男性不育弱精子症的作用机制。方法:采用Percoll梯度离心法优选具有正常生理功能的精子作为正常精子模型,将精子悬液分为正常组,模型组,阳性药对照组(维生素C组)和菟丝子小、中、大剂量组。应用次黄嘌呤-黄嘌呤氧化酶(HX-XO)体系产生活性氧,在有氧环境下,ROS、不同含量(0.125,0.25,0.5 g.mL-1)的菟丝子水提物与精子悬液共同孵育后,计算精子顶体完整率,透射电镜观察精子顶体和超微结构,并与已知的抗氧化剂维生素C对照。结果:终含量0.125,0.5 g.mL-1的菟丝子水提物对精子顶体和超微结构的保护作用与维生素C(0.25 mg.mL-1)无明显差别(P均>0.05),0.25 g.mL-1的菟丝子水提物的作用明显优于维生素C(P<0.001)。结论:适宜含量的菟丝子水提物对ROS造成的精子膜、顶体结构和精子线粒体功能损伤具有明显的保护作用。
Objective： To study the oxidation damage of active oxygen （ROS） to human sperm acrosome and uhrastructure, and study the function mechanism about cuscutajaponica treating male＇s infertility and asthenoospermia. Method： By using the Percoll gradient centrifugation, the sperm with normal physiological function were selected for the normal sperm model, and the sperm suspension were divided into the normal group, the model group, the positive control group （Vitamin C group）, and the lugh, the median and the low dose groups of C. japonica. The ROS made from hypoxanthine-xanzine xanzine（HX-XO） and different content（0.125, 0.25, 0.5 g ·mL^-1） of extract were incubated with sperm in the oxygen environment. The acrosomic integrity rate were calculated and the sperm acrosome and ultrastructure were observed. Result： The content （ 0.125, 0.5 g·mL^-1 ） of extract had no obvious difference as compared with Vitamin C （ 0.25 mg·mL^1- ） in protecting the acrosome and ultrastructure, but the content（0.25 mg·mL^-1 ） of extract was significantly better than Vit C（ P 〈 0.001）. Conclusion： The suitable content of extract from C. japonica can significantly protect the sperm membrane, the acosomic structure and the mitochondrion function from the damage caused by ROS.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第5期422-425,共4页
China Journal of Chinese Materia Medica
基金
浙江省中医药科研基金项目(2003C070)
关键词
菟丝子
活性氧
精子
顶体
超微结构
脂质过氧化作用
Cuscuta japonica
active oxygen
sperm
acrosome
ultrastructure
lipin peroxidation function
