摘要
目的 探讨钙通道拮抗剂在体外对人精子运动参数的影响。方法 将不同类型的钙通道拮抗剂在体外与生育男性经上游优化处理的精子分别共同孵育 10、2 0、3 0、60min ,与正常组进行对照研究。采用计算机辅助精子分析系统检测人精子运动参数 (精子活率、精子前向运动百分率、畸形率、平均路径速度、精子侧摆幅度和摆动幅度 )。结果 硝苯地平 (10 μmol/L)在体外对精子活率 (P <0 .0 1)、精子前向运动百分率 (P <0 .0 1)、精子平均路径速度 (P <0 .0 0 1)、精子侧摆幅度 (P <0 .0 1)和摆动幅度 (P <0 .0 1)等指标的差异有非常显著性。结论 人精子细胞中存在L 型电压依赖性钙通道 ,且L 型钙通道拮抗剂可导致男性不育。
Objective To investigate the effects of calcium channel blocker (CCB) on human sperm motility in vitro.Methods Sperms were aseptically obtained by masturbation and prepared by swim up technique to produce a spermatozoa solution of high motility from 20 healthy fertile men.The spermatozoa solution was incubated with the different types of CCBs at 37 ℃ in vitro.Motive parameters were measured by computer aided sperm analysis (CASA).Measurements were carried out at 10,20,30 and 60 min in all specimens,including live rate,progressive motility,malformed rate,VAP,ALH and WOB.Results Nifedipine (10 μmol/L) could significantly affect human spermatozoa motility incubated in vitro ( P <0.001).Conclusion There is the L type voltage depended calcium channels (VDCC) in human spermatozoa.L type CCB can cause reversible male infertility.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第6期725-727,共3页
Chinese Journal of Experimental Surgery
