摘要
目的 :通过分析Percoll梯度离心法处理前后精子相关功能指标 ,评价该法在精子体外处理中的作用。 方法 :采用多次曝光摄影技术、瑞 姬氏染色法、低渗肿胀实验 ,观察处理前后精子运动速度、顶体完整率和低渗肿胀率的变化。 结果 :2 5例不育病人精液标本经Percoll梯度离心法处理前后 ,精子运动速度 (2 0 .5 6± 8.13μm/s和 2 5 .18± 8.30 μm/s ,P <0 .0 5 )、顶体完整率 (6 1.2 0 %± 2 1.11%和 70 .36 %± 17.70 % ,P <0 .0 1)和低渗肿胀率 (6 8.2 8%±17.33%和 76 .76 %± 17.10 % ,P <0 .0 5 )均有显著差异。 结论 :精液标本经Percoll梯度离心法处理后 ,各项精子功能指标均较处理前有明显改善 ,该法可广泛应用于辅助生殖医学中。
Objectives: To evaluate the sperm functional parameters with Percoll gradient centrifugation. [WT5”HX]Methods:[WT5”BZ] The changes of sperm velocity, percentage of acrosomal integrity and percentage of swollen sperm before and after Percoll gradient centrifugation were evaluated by multiple exposure photography technique, Wright Giemsa staining and hypo osmotic swelling test (HOST). [WT5”HX]Results: [WT5”BZ]The semen samples from 25 infertile patients were treated by Percoll gradient centrifugation. Before and after the treatment, the changes of sperm velocity (from 20.56±8.13μm/s to 25.18±8.30μm/s; P <0.05 ), percentage of acrosomal integrity (from 61.20%±21.11% to 70.36%±17.70%; P <0.01) and percentage of swollen sperm (from 68.28% ±17.33% to 76.76%±17.10%; P <0.05) were statistically significant. [WT5”HX] Conclusions: [WT5”BZ] The spermatic functional parameters were distinctly improved after Percoll gradient centrifugation. Consequently, Percoll gradient centrifugation technique may be widely applied in reproductive medicine.
出处
《中华男科学杂志》
CAS
CSCD
2000年第3期161-163,共3页
National Journal of Andrology
关键词
Percoll梯度离心法
精子
运动速度
顶体完整率
Percoll gradient centrifugation
Sperm
Velocity
Percentage of swollen sperm
Percentage of acrosomal integrity
