摘要
目的:对毕赤酵母重组菌GS115-Ch-Glu菌种进行发酵脱毒条件的优化,为大规模发酵重组毕赤酵母获得高表达目的蛋白奠定基础。方法:以毕赤酵母重组菌为实验菌株,分别以葡萄糖、乳糖、玉米淀粉、蛋白胨、甘油、玉米淀粉+甘油为碳源,研究不同碳源对菌株细胞生长的影响。结果:毕赤酵母重组菌的最适碳源为玉米淀粉,最适浓度为2.5%。
Objective：Optimize the fermentation detoxication conditions on recombinant strains of pichia GS115 -Ch - Glu in order to lay the foundation for large - scale fermentation of recombinant Pichia pastoris to obtain high expression of the target protein. Methods ： Make the Pichia pastoris recombinant bacteria as the test strains ,and make glucose, lactose, corn starch, peptone, glycerin, corn starch ＋ glycerol as carbon source respectively, then study the effects of different carbon sources on the growth of the cells. Results： The Pichia pastoris recombinant strain optimum carbon source is corn starch and the optimal concentration is 2.5%
出处
《广东轻工职业技术学院学报》
2012年第4期11-14,共4页
Journal of Guangdong Industry Polytechnic
基金
广东轻工职业技术学院自然科学项目(KJ201107)
关键词
重组毕赤酵母
碳源
摇瓶发酵
优化
recombinant pichiapastoris
carbon source
shake
flask fermentation
optimization
