摘要
目的 :观察葛根素 (Pue)对肝脏缺血再灌注损伤的保护作用。方法 :建立大鼠全肝缺血再灌注模型 ,比较Pue处理对再灌注血ET 1、NO和ALT水平 ,肝组织中MDA、细胞总钙含量和SOD活性 ,以及肝组织光镜下的形态学改变。结果 :Pue处理可使血NO含量、肝组织SOD活性明显增高 ;而血ET 1、ALT含量及肝组织MDA、细胞总钙含量明显降低。肝组织形态损伤也明显减轻。Pue保护效应与剂量大小明显相关。结论 :葛根素对肝缺血再灌注损伤有显著的药物预适应作用 ,其主要机制是清除氧自由基、减轻细胞内Ca+ +
Objective: To evaluate the protective effects of puerarin on hepatic injury during ischemia/reperfusion procedure, and to explore the pharmacological mechanism of puerarin. Methods: 40 SD rats were divided randomly into 4 groups (n=10): I/R groups (A), puerarin group with low dose (20mg/kg) (B1), puerarin group with moderate dose (40mg/kg) (B2), puerarin group with high dose (80mg/kg) (B3). Hepatic ischemia reperfusion models were setted up. At the end of reperfusion, the NOx?ET?ALT levels in plasma of each group were detected, as well as the SOD?MDA and Ca ++ levels in tissue were observed. Results: In puerarin groups the serum level of NO were higher than that in I/R group (P<0.01); while plasma levels of ET?ALT were lower (P<0.01). The SOD level of hepatic tissue were higher in puerarin groups than that in I/R group (P<0.01); as well as MDA?Ca ++ levels were lower (P<0.01). All these changes were related to the dose of the puerarin. And abnormal morphological changes of liver during I/R procedure were ameliorated remarkably in puerarin groups. Conclusions: Puerarin attenuates the hepatic injury of ischemia/reperfusion by inhibiting the production of oxygenic free radicals; preventing hepatocellular Ca ++ overload and improving the function of microcirculation. And the protective effect was related to the dose of the puerarin.
出处
《中药药理与临床》
CAS
CSCD
2004年第4期15-16,共2页
Pharmacology and Clinics of Chinese Materia Medica
