摘要
以反相离子对色谱法测定头孢三嗪的含量。色谱柱为ＷａｔｅｒｓＮｏｖａ－ＰａｋＣ＿８５μｍ（３．９ｍｍ×１５０ｍｍ），流动相为乙腈－复合缓冲液（２８：７２）；反离子为四丁基溴化铵；检测波长为２７０ｎｍ。在浓度３４．１～７９．６μｇ／ｍｌ的范围内，浓度与峰面积呈良好的线性关系。本法回收率为９９．４％，柱效为４０８２理论塔板数，拖尾因子为１．２５，重复进样的相对标准差为０．３１％。
A reverse-phase ion-pair HPLC for the quantitative determination of ceftriaxone was developed.The column(3.9mm×150mm)was packed with Nova-Pak C_8(5μm).The mobile phase was acetonitrile-buffer solution(Dissolve 3.4g of tetrabutylammonium bromide in 500ml water,add 74ml of pH7. 0 phosphate buffer solution and 6.7ml of pH5.0 citrate buffer solution,and add water to make 1000ml)(28:72).The counter ion was tetrabutylammonium bromide,The detector wave length was 270nm. The quantitative analysis was performed using external standard.This method was fast with good linearity and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1995年第6期25-27,共3页
Chinese Journal of Pharmaceutical Analysis
