摘要
AIM： To study the expression of vascular endothelial growth factor A （VEGF-A） and VEGF-C and to determine whether the presence of VEGF-A and VEGF-C was associated with the clinicopathologic characteristics of pancreatic cancer. METHODS： VEGF-A and VEGF-C mRNA transcripts were examined by Northern blot in 6 human pancreatic cancer cell lines and 8 normal pancreatic tissues and 8 pancreatic carcinoma specimens. The expression of VEGF-A and VEGF-C proteins was examined by Western blot in the tested cell lines and by immunohistochemical stain in 50 pancreatic carcinoma samples. RESULTS： VEGF-A and VEGF-C mRNA transcripts were present in all the 6 human pancreatic cancer cell lines. Immunoblotting revealed the presence of VEGF-A and VEGF-C proteins in all the cell lines. Northern blot analysis of total RNA revealed 3.0-fold and 3.6-fold increase in VEGF-A and VEGF-C mRNA transcript in the cancer samples, respectively. Immunohistochemical analysis confirmed the expression of VEGF-A and VEGF-C in cancer cells within the tumor mass. Immunohistochemical analysis of 50 pancreatic cancer tissue samples revealed the presence of VEGF-A and VEGF-C immunoreactivity in 50% and 80% of the cancer tissue samples, respectively. The presence of VEGF-A in these cells was associated with larger tumor size and enhanced local spread （x^2= 6.690, P= 0.035〈0.05） but was not associated with decreased patient survival. However, the presence of VEGF-C in the cancer cells was associated with increased lymph node metastasis （x^2= 5.710, P= 0.017〈0.05）,but was not associated with decreased patient survival. There was no correlation between the expression of VEGF-A and VEGF-C in the same cancer cells. CONCLUSION： VEGF-A and VEGF-C are commonly overexpressed in human pancreatic cancer and may contribute to tumor growth and lymph node metastasis, There is no relationship between the expression of VEGF-A and VEGF-C in pancreatic cancer.
瞄准：学习脉管的内皮生长因素 A 的表示(VEGF -- 一) 并且 VEGF-C 并且决定是否 VEGF 的存在 -- A 和 VEGF-C 与胰腺的癌症的临床病理特征被联系。方法：VEGF -- A 和 VEGF-C mRNA 抄本被北污点在 6 根人的胰腺的癌症房间线和 8 正常胰腺的纸巾和 8 个胰腺的癌标本检验。VEGF 的表示 -- A 和 VEGF-C 蛋白质被西方的污点在测试房间线检验，由免疫，在 50 胰腺的癌的组织化学的污点取样。结果：VEGF -- A 和 VEGF-C mRNA 抄本在所有 6 根人的胰腺的癌症房间线是在场的。Immunoblotting 揭示了 VEGF 的存在 -- 在所有房间的 A 和 VEGF-C 蛋白质排队。全部的 RNA 的北污点分析在 VEGF 揭示了 3.0 褶层和 3.6 褶层增加 -- 在癌症的 A 和 VEGF-C mRNA 抄本分别地取样。Immunohistochemical 分析证实了 VEGF 的表示 -- 在在肿瘤以内的癌症房间的 A 和 VEGF-C 集中。50 件胰腺的癌症织物样品的 Immunohistochemical 分析揭示了 VEGF 的存在 -- 在 50% 癌症织物和 80% 的 A 和 VEGF-C 免疫反应分别地取样。VEGF 的存在 -- 在这些房间的 A 与更大的肿瘤尺寸被联系并且提高了本地传播(c2 = 6.690， P = 0.035 <0.05 ) 但是没与减少的耐心的幸存被联系。然而，在癌症房间的 VEGF-C 的存在与增加的淋巴节点转移被联系(c2 = 5.710， P = 0.017 < 0.05 ) ，但是没与减少的耐心的幸存被联系。在 VEGF 的表示之间没有关联 -- 在一样的癌症房间的 A 和 VEGF-C。结论：VEGF -- A 和 VEGF-C 是通常在上在人的胰腺的癌症表示了并且可以贡献肿瘤生长和淋巴节点转移。在 VEGF 的表示之间没有关系 -- 在胰腺的癌症的 A 和 VEGF-C。
基金
Supported by grant from Ministry of Education of China, No. 2002247
