摘要
AIM- To immunohistochemicaUy examine micrometastasis and VEGF-C expression in hilar bile duct carcinoma （HBDC） and to evaluate the clinical significance of the results. METHODS： A total of 361 regional lymph nodes from 25 patients with node-negative HBDC were immunostained with an antibody against cytokeratins 8 and 18 （CAM 5.2）, and immunohistochemical staining of VEGF-C was performed in 34 primary resected tumors. RESULTS： Lymph node micrometastasis was detected in 6 （24%） of the 25 patients and 10 （2.8%） of the 361 lymph nodes. Patients with micrometastasis showed significantly poorer survival rates than those without （P= 0.025）. VEGF-C expression was positive in 17 （50%） of 34 HBDC, and significantly correlated with lymph node metastasis （P=0.042） and microscopic venous invasion （P=0.035）. CONCLUSIONS： It is suggested that immunohistochemically detected lymph node micrometastasis has an impact on the outcome of HBDC. VEGF-C expression is highly correlated with lymph node metastasis in HBDC and might therefore be a useful predictor.
瞄准：组织化学地在门胆汁管癌(HBDC ) 并且到检验微转移和 VEGF-C 表示到免疫评估结果的临床的意义。方法：从有节点否定的 HBDC 的 25 个病人的 361 个地区性的淋巴节点的一个总数是对 cytokeratins 与抗体染色的免疫 8 和 18 (凸轮 5.2 ) ，并且免疫 VEGF-C 的组织化学的染色在 34 个主要 resected 肿瘤被执行。结果：淋巴节点微转移在 6 被检测(24%) 25 个病人并且 10 (2.8%) 361 个淋巴节点。没有，有微转移的病人比那些显示出显著地更差的幸存率(P = 0.025 ) 。VEGF-C 表示是积极的在里面(50%) 17 与淋巴节点转移 34 HBDC，并且显著地相关(P = 0.042 ) 并且显微镜的静脉的侵略(P = 0.035 ) 。结论：免疫组织化学地检测了微转移影响的淋巴节点，这被建议 HBDC 的结果。VEGF-C 表示高度在 HBDC 与淋巴节点转移被相关并且可能因此是一个有用预言者。
